Latent KSHV infection increases the vascular permeability of human endothelial cells

Supplemental materials for: Guilluy et al

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• Figure S1. Latent KSHV infection of endothelial cells (JPG, 110 KB) -
  (A) We used a recombinant virus to infect endothelial cells (KSHV-HUVEC), which expresses GFP under the EF-1alpha promoter and RFP under the control of a viral lytic promoter. Immunofluorescence images of these cells show that each and every cell is infected with the virus (as scored by GFP expression) and the cells do not exhibit RFP expression. The reactivated KSHV-infected 293 cells (KSHV-293*) serve as a positive control for lytic RFP expression. (B) RT-PCR was performed to detect viral gene expression in the KSHV-HUVEC. Uninfected HUVEC and reactivated KSHV-infected 293 cells (KSHV-293*) were used as negative and positive controls, respectively. RT-PCR was performed with primers against viral genes, vGPCR, Rta, K1 and LANA. RT-PCR was also performed with primers for human GAPDH as a control.