Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Supplementary Movie S1 Movie of a nsk1-gfp sid4-tdtomato cell from figure 3C. Nsk1-gfp (green) localises to kinetochores at anaphase onset and colocalises with SPBs (red) during anaphase B.
- Movie02 - Supplementary Movie S2 Movie of a nsk1-gfp sid4-tdtomato ��dlc1 cell from figure 6C. Nsk1-gfp (green) co-localises transiently with SPBs (red) during anaphase B.
- Movie03 - Supplementary Movie S3 Movie of a class I anaphase in a ��nsk1 fta3-gfp sid4-tdtomato cell from figure 7B. Kinetochores (green) remain associated with SPBs (red) throughout anaphase B.
- Movie04 - Supplementary Movie S4 Movie of a class II anaphase in a ��nsk1 fta3-gfp sid4-tdtomato cell from figure 7B. A single kinetochore detaches from a SPB in late anaphase B.
- Movie05 - Supplementary Movie S5 Movie of a class III anaphase in a ��nsk1 fta3-gfp sid4-tdtomato cell from figure 7B. A single kinetochore detaches from a SPB in early anaphase B and is not recaptured.
- Movie06 - Supplementary Movie S6 Movie of a class IV anaphase in a ��nsk1 fta3-gfp sid4-tdtomato cell from figure 7B. One kinetochore is detached from an SPB as the movie starts. As anaphase B progresses the remaining two kinetochores sequentially detach from the same SPB.
- Movie07 - Supplementary Movie S7 Movie of a ��nsk1 fta3-gfp sid4-tdtomato cell from figure 8A. Kinetochores (green) are clustered close to SPBs (red) on entry into mitosis
- Movie08 - Supplementary Movie S8 Movie of a ��nsk1 fta3-gfp sid4-tdtomato cell from figure 8A. An unclustered kinetochore is retrieved to the SPB before anaphase onset but the length of prometaphase and metaphase is extended.
- Movie09 - Supplementary Movie S9 Movie of a ��nsk1 ��dis2 fta3-gfp sid4-tdtomato cell from figure 8A. An unclustered kinetochore is not captured and spindle collapses after prolonged delay in prometaphase.