AccD6, a Key Carboxyltransferase Essential for Mycolic Acid Synthesis in Mycobacterium tuberculosis, Is Dispensable in a Nonpathogenic Strain

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Table S1, plasmids used in this study
  PDF file, 3.3MB.
• Supplemental file 2 - Table S2, primers used for PCR amplification
  PDF file, 4.3MB.
• Supplemental file 3 - Fig. S1, fatty acid/mycolic acid biosynthesis in mycobacteria
  PDF file, 1.8MB.
• Supplemental file 4 - Fig. S2, PCR-based genotyping of the M. tuberculosis accD6 mutants
  PDF file, 3.2MB.
• Supplemental file 5 - Fig. S3, PCR analysis of M. smegmatis ΔaccD6 DCO mutants
  PDF file, 2.8MB.
• Supplemental file 6 - Fig. S4, 2D-TLC lipid analysis and cell wall permeability test of ΔaccD6 mutant versus wild-type M. smegmatis
  PDF file, 7.3MB.