Deletion of the Aconitase Gene in Corynebacterium glutamicum Causes Strong Selection Pressure for Secondary Mutations Inactivating Citrate Synthase

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Table S1, oligonucleotides used in this study; Fig. S1, growth of C. glutamicum wild type and C. glutamicum Δcan-1 on CGXII minimal medium with L-glutamine or L-glutamate supplementation; Fig. S2, complementation studies with C. glutamicum Δcan-1; section SD, proteome analysis of C. glutamicum Δcan-1; Fig. S3, comparative 2D gel analysis of C. glutamicum Δcan-1 and wild type under iron starvation and iron excess; Table S2, proteins differentially expressed in C. glutamicum Δcan-1 and the wild type; section SE, transcriptome analysis of C. glutamicum wild type and C. glutamicum Δcan-1, using DNA microarrays; Table S4, genes with an mRNA level changed ≥10-fold in C. glutamicum Δcan-1 compared to the wild type under standard iron conditions; Table S6, genes with an mRNA level changed ≥10-fold in C. glutamicum Δcan-1 compared to the wild type under iron starvation; Fig. S4, experimental design for testing the influence of suddenly increased cytoplasmic citrate levels on C. Glutamicum
    PDF file, 1.3MB.
  • Supplemental file 2 - Table S3, DNA microarray results for the comparison of the Δcan-1 mutant with the wild type after growth in glucose minimal medium with 35 μM iron
    MS Excel file, 157K.
  • Supplemental file 3 - Table S5, DNA microarray results for the comparison of the Δcan-1 mutant with the wild type after growth in glucose minimal medium with 1 μM iron
    MS Excel file, 114K.
  • Supplemental file 4 - Table S7, DNA microarray results for wild type with plasmid pAN6 20 min after citrate addition versus before citrate addition
    MS Excel file, 55K.
  • Supplemental file 5 - Table S8, DNA microarray results for wild type with plasmid pAN6-citH 20 min after citrate addition versus before citrate addition
    MS Excel file, 353K.