Imaging and Analysis of Pseudomonas aeruginosa Swarming and Rhamnolipid Production

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Time-lapse series of P. aeruginosa swarming; "all" channel is merged display of GFP-tagged cells (GFP channel) and Nile red-stained rhamnolipid (Nile red channel); frames were acquired 22 min apart for 50 h (Movie S1).
    AVI Video file, 1.4 MB.
  • Supplemental file 2 - Time-lapse series of coswarming experiment with P. aeruginosa (green) and S. Typhimurium (red); frames were acquired 25 min apart for 60 h (Movie S2).
    AVI Video file, 2.5 MB.
  • Supplemental file 3 - Rhamnolipid congeners for different P. aeruginosa strains from liquid culture growth in FAB plus glucose (Table S1).
    Zipped PDF file, 31K.
  • Supplemental file 4 - Intensity fluorescent response of GFP-P. aeruginosa (Fig. S1).
    TIF Image file, 2.8 MB.
  • Supplemental file 5 - Intensity fluorescent response of Nile red-stained rhamnolipid (Fig. S2).
    TIF Image file, 419K.
  • Supplemental file 6 - Fluorescence imaging of cells stained with Syto24 and rhamnolipid stained with Nile red for swarms of different P. aeruginosa strains after 48 h on FAB-glucose medium with 0.45% noble agar (Fig. S3).
    TIF Image file, 8 MB.
  • Supplemental file 7 - Comparative planktonic and surface rhamnolipid production for strains of P. aeruginosa (Fig. S4).
    TIF Image file, 3 MB.