Supplemental Data
Files in this Data Supplement:
- Supplemental Tables 1-3 and Figures 1-4 (.pdf, 702 KB) - Table S1. Plasmids used in this study Table S2. Primers used in this study Table S3. Cys pairs within passenger domains of selected ATs Fig. S1. Alignment demonstrating that D2A and the conserved Cys residues are present only in cytotoxic SPATE proteins Fig. S2. A three-dimensional model of the Pet passenger domain showing the positions of the Cys pair in Pet6aa to Pet48aa Fig. S3. A FLAG epitope tag in the C-terminus of the Pet passenger domain does not abolish secretion Fig. S4. A schematic description of stalled versus secreted passenger domains