VSX1 Primer sequences
|
V1F
|
5′-GCAGCCCAATCCTATAAAGC-3′
|
687
|
1–20
|
V1R
|
5′-GATTACCGGACGTGGAGA-3′
|
|
469–486
|
V2F
|
5′-AAGTCCTCTTCTTCTTTCTGTGCCATC-3′
|
800
|
2610–2637
|
V2R
|
5′-AAGGGACTGCTGATTGGCTCACTG-3′
|
|
3386- 3409
|
V3F
|
5′-ATCATGCTCGGGAGAGAAGA-3′
|
487
|
4178–4197
|
V3R
|
5′-AAAATGAGGCAACCATCCAG-3′
|
|
4639–4660
|
V4F
|
5′-CCAATGCCAATCACTGTGTC3′
|
306
|
5366–5385
|
V4R
|
5′-CCCAGAGTCCTGCCAACTTA-3′
|
|
5652–5672
|
V5F
|
5′-AGGAAGTGAAGATAAGTTGGCAG-3′
|
470
|
5640–5662
|
V5R
|
5′-TAAAGTGCCATTAAGGAACCG-3′
|
|
6110–6090
|
V6F
|
5′-AACGGTTCCTTAATGGCACTT-3′
|
301
|
6088–6109
|
V6R
|
5′-TTGAAATATCCAAGGCCAAGTT-3′
|
|
6367–6388
|
V7F
|
5′-ATCATAGTGAAGACTCCATACAGACA-3′
|
424
|
6312–6337
|
V7R
|
5′-AGCCCTCACAATGAGCAGTT-3′
|
|
6964–6984
|
V8F
|
5′-GAGGCAGCATCTCAGGACTT-3′
|
534
|
9834–9854
|
V8R
|
5′-AGGTGTGAGGTACAGGTCCAA-3′
|
|
9321–9340
|
V9F
|
5′-GCTCAGGTAGCATTGTTCTGC-3′
|
610
|
10272–10292
|
V9R
|
5′-TGATGGAAGGAGAGGAGAAGG-3′
|
|
10861–10881
|
ARMS primers
|
VSM
|
5′-AGTCTGGCAGCGAGATGTAGC-3′
|
236
|
4392–4411
|
VSWT
|
5′-ACTGCATCCCGCTGCCAGACT-3′
|
|
43911–4411
|
VSF
|
5′-GGATCATGCTCGGGAGAGAAGA-3′
|
|
4176–4197
|
SOD1 primer sequences
|
S1F
|
5′-CTCCACATTTCGGGGTTCT-3′
|
450
|
4850–4868
|
S1R
|
5′-ACCCGCTCCTAGCAAAGGT-3′
|
|
5281–5292
|
S2F
|
5′-CCATCTCCCTTTTGAGGACA-3′
|
426
|
8965–8985
|
S2R
|
5′-CGACAGAGCAAGACCCTTTC-3′
|
|
9371–9390
|
S3F
|
5′-TGATGCAGGTCAGCACTTTC-3′
|
344
|
11717–11736
|
S3R
|
5′-AAAAGCATTCCAGCATTTGG-3′
|
|
12041–12060
|
S4F
|
5′-CCATCTTTCTTCCCAGAGCA-3′
|
386
|
12810–12840
|
S4R
|
5′-GAAACCGCGACTAACAATCAA-3
|
|
12454–12473
|
S5F
|
5′-TTTGGGTATTGTTGGGAGGA-3′
|
675
|
13780–13799
|
| S5R |
5′-TCTGTTCCACTGAAGCTGTTT-3′ |
|
14334–14355 |
