Files in this Data Supplement:
Fig. S1. Temporal and spatial expression of the axial mesoderm markers Gsc and Foxa2 from LS to LHF stages. (A) Gsc and (B) Foxa2 expression in lateral (left) and frontal (right) views. Scale bar: 170 µm.
Fig. S2. The mouse embryo model and the 3D reconstruction. (A) A 5- to 6-somite mouse embryo, embedded in epoxy resin after 24 hours of culture from the LSEB stage. (B,C) This embryo was used to build the 3D computer reconstruction of the head as shown in (B) lateral view and (C) frontal view. Scale bar: 120 µm.
Fig. S3. Still frames from the animation (Movie 1) showing the spatial distribution of clones with respect to the extracted ectoderm surface in 3D and a transition to the 2D flat map (dorsal view). (A) Clone domains and the ectoderm surface shown shaded and partially transparent. (B) Clone domains and the ectoderm surface represented as a triangulated mesh. (C-E) Clone domains replaced by spheres (situated at the cell centres of mass) as the mesh flattens to the interior of the boundary defined by its intersection with the cutting plane. (F,F′) The surface curvature and anatomical domains superimposed on the flattened mesh (F) and enlarged (F′).
Movie 1. Animation of the spatial distribution of the clones with respect to the extracted ectoderm surface in 3D and a transition to the flat map representation shown in Fig. 8. In the first rotation the ectoderm surface is shown shaded and partially transparent. In the second, the triangulated mesh representation used is made explicitly visible. The clone domains are then replaced by spheres (situated at the cell centres of mass) and the mesh is then shown flattening to the interior of the boundary defined by its intersection with the cutting plane. Finally, once flat, the surface curvature and anatomical domains are superimposed.
