Molecular Characterization of Vegetative Incompatibility Genes That Restrict Hypovirus Transmission in the Chestnut Blight Fungus Cryphonectria parasitica

Supporting Information for Choi et al., 2011

Supporting Information

  • Supporting Information - Figures S1-S11 and Tables S1 and S2 (PDF, 1 MB)
  • Figure S1 - Amino acid alignment of candidate vic2 alleles in strain EP155 (vic2-2) and in strain EP146 (vic2-1) performed using MegAlign in Lasergene (DNASTAR Inc. Madison WI)(PDF, 84 KB)
  • Figure S2 - Amino acid alignment of the Sec9-like protein candidate vic2a alleles in strain EP155 (vic2a-2) and EP146 (vic2a-1) (PDF, 72 KB)
  • Figure S3 - Alignment of linkage group LGI of the C. parasitica genetic linkage map generated by Kubisiak TL, Milgroom MG (2006 Fungal Genetics and Biology 43: 453-463) that contains the position of the vic4 genetic locus with Scaffold 4 of version 2 of the C. parasitica genome sequence (PDF, 64 KB)
  • Figure S4 - Alignment of linkage group LGXIV of the C. parasitica genetic linkage map generated by Kubisiak and Milgroom (2006 Fungal Genetics and Biology 43: 453-463) that contains the position of the vic6 genetic locus with Scaffold 3 of version 2 of the C. parasitica genome sequence (PDF, 64 KB)
  • Figure S5 - Amino acid alignment of vic6 alleles in strain EP155 (vic6-2) and in strain EP146 (vic6-1) (PDF, 64 KB)
  • Figure S6 - Amino acid alignment of alleles pix6-1 and pix6-2 in strains EP146 and EP155, respectively (PDF, 72 KB)
  • Figure S7 - Alignment of linkage group LGVI of the C. parasitica genetic linkage map generated by Kubisiak and Milgroom (2006 Fungal Genetics and Biology 43: 453-463) that contains the position of the vic7 genetic locus with Scaffold 6 of version 2 of the C. parasitica genome sequence (PDF, 64 KB)
  • Figure S8 - Amino acid alignment of candidate vic7 alleles in strain EP155 (vic7-2) and in strain EP146 (vic7-1) (PDF, 80 KB)
  • Figure S9 - Agarose gel migration of candidate vic allele-specific PCR fragments (PDF, 108 KB)
  • Figure S10 - Colony morphology for disruption strain DK80 Δvic6-2 (middle panel) and replacement strain DK80 vic6-2::vic6-1 (right panel) in which the disrupted vic6-2 allele was replaced with an intact vic6-1 gene (PDF, 184 KB)
  • Figure S11 - Mycelial incompatibility assay for strain DK80 disrupted in pix6-2 and vic6-2 (PDF, 104 KB)
  • Table S1 - Oligonucleotide primers used in PCR reactions (PDF, 60 KB)
  • Table S2 - Single Sequence Repeat (SSR) linkage markers used in this study (PDF, 52 KB)