Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Effect of bead beating time and cooling period on sample temperature (Fig. S1); effect of bead beating cycles on LDH activity and protein content (Fig. S2); reproducibility of Vmax measurements in chemostat cultures (Fig. S3); enolase activity measuring NADH or PEP absorbance (Table S1); rationale for the choice of buffer and statistical calculations (Document S1).
MS Word document, 51K. - Supplemental file 2 - Standard operating protocol for the preparation of cell extracts and for the measurement of enzyme activities (Document S2).
MS Word document, 119K. - Supplemental file 3 - Detailed description of composition and reagents of the standardized enzyme assays (Document S3).
MS Excel file, 140K.