A Naturally Occurring Single-Residue Mutation in the Translocator Domain of Neisseria meningitidis NhhA Affects Trimerization, Surface Localization, and Adhesive Capabilities

Supplemental Material

Files in this Data Supplement:

• Supplemental Figure 1 - Table S1. Strains and plasmids used in this study.
  MS Word document, 45K.
• Supplemental Figure 2 - Table S2. Primers used in this study.
  MS Word document, 134K.
• Supplemental Figure 3 - Fig. S1. Growth properties of wild-type and recombinant strains.
  MS Word document, 99K.
• Supplemental Figure 4 - Fig. S2. Analysis of NhhA production in a panel of N. meningitidis non-serogroup B strains.
  MS Word document, 493K.
• Supplemental Figure 5 - Fig. S3. Quantitative analysis of degQ transcript levels in MC58 and M1390 strains.
  MS Word document, 39K.
• Supplemental Figure 6 - Fig. S4. Coomassie staining of OMP preparations subjected to SDS-PAGE and analyzed by Western blotting in Fig. 6A for detection of NhhA.
  MS Word document, 385K.