Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movies S1-S4. Self streaming of WT-cells (Movie S1), ctxA--cells (Movie S2), ctxB--cells (Movie S3) and ctxA-/B--cells (Movie S-4). Cells were placed on plates in starvation buffer and cells streaming and aggregating were recorded with a stereo microscope. Images were captured at 1 frame/min and are played back at 30 frames/s. Movies S1-S4 correspond to Figure 4A.
- Movie02 - Movies S1-S4. Self streaming of WT-cells (Movie S1), ctxA--cells (Movie S2), ctxB--cells (Movie S3) and ctxA-/B--cells (Movie S-4). Cells were placed on plates in starvation buffer and cells streaming and aggregating were recorded with a stereo microscope. Images were captured at 1 frame/min and are played back at 30 frames/s. Movies S1-S4 correspond to Figure 4A.
- Movie03 - Movies S1-S4. Self streaming of WT-cells (Movie S1), ctxA--cells (Movie S2), ctxB--cells (Movie S3) and ctxA-/B--cells (Movie S-4). Cells were placed on plates in starvation buffer and cells streaming and aggregating were recorded with a stereo microscope. Images were captured at 1 frame/min and are played back at 30 frames/s. Movies S1-S4 correspond to Figure 4A.
- Movie04 - Movies S1-S4. Self streaming of WT-cells (Movie S1), ctxA--cells (Movie S2), ctxB--cells (Movie S3) and ctxA-/B--cells (Movie S-4). Cells were placed on plates in starvation buffer and cells streaming and aggregating were recorded with a stereo microscope. Images were captured at 1 frame/min and are played back at 30 frames/s. Movies S1-S4 correspond to Figure 4A.
- Movie05 - Movies S5-S8. Micropipette Assay of Individual Cell Chemotaxis. WT-cells (Movie S-5), ctxA--cells (Movie S-6), ctxB--cells (Movie S-7) and ctxA-/B--cells (Movie S-8) were exposed to a micropipette containing 10 �M cAMP and the migration of individual cells visualized by time-lapse confocal microscopy. Images were captured at 1 frame/10 s and are played back at 10 frames/s. Movies S5-S8 correspond to Figure 4C.
- Movie06 - Movies S5-S8. Micropipette Assay of Individual Cell Chemotaxis. WT-cells (Movie S-5), ctxA--cells (Movie S-6), ctxB--cells (Movie S-7) and ctxA-/B--cells (Movie S-8) were exposed to a micropipette containing 10 �M cAMP and the migration of individual cells visualized by time-lapse confocal microscopy. Images were captured at 1 frame/10 s and are played back at 10 frames/s. Movies S5-S8 correspond to Figure 4C.
- Movie07 - Movies S5-S8. Micropipette Assay of Individual Cell Chemotaxis. WT-cells (Movie S-5), ctxA--cells (Movie S-6), ctxB--cells (Movie S-7) and ctxA-/B--cells (Movie S-8) were exposed to a micropipette containing 10 �M cAMP and the migration of individual cells visualized by time-lapse confocal microscopy. Images were captured at 1 frame/10 s and are played back at 10 frames/s. Movies S5-S8 correspond to Figure 4C.
- Movie08 - Movies S5-S8. Micropipette Assay of Individual Cell Chemotaxis. WT-cells (Movie S-5), ctxA--cells (Movie S-6), ctxB--cells (Movie S-7) and ctxA-/B--cells (Movie S-8) were exposed to a micropipette containing 10 �M cAMP and the migration of individual cells visualized by time-lapse confocal microscopy. Images were captured at 1 frame/10 s and are played back at 10 frames/s. Movies S5-S8 correspond to Figure 4C.
- Movie09 - Movie S9. Localization of GFP-Myosin II Expressed in ctxA-/B--cells. GFP-myosin II accumulates at the contractile ring and cleavage furrow in dividing cell visualized by time-lapse confocal microscopy. Left, phase-contrast; right, GFP-myosin II. Images were captured at 1 frame/10 s and are played back at 5 frames/s. Movie S9 corresponds to Figure 8B (middle row).
- Movie10 - Movies S10-S11. Self Streaming of ACA-YFP/ctxA-/B--cells (Movie S10) and cAR1-YFP/ctxA-/B--cells (Movie S11). Live images recorded as described for Movies S-1-S4. Movies S10-S11 correspond to Figure 9E.
- Movie11 - Movies S10-S11. Self Streaming of ACA-YFP/ctxA-/B--cells (Movie S10) and cAR1-YFP/ctxA-/B--cells (Movie S11). Live images recorded as described for Movies S-1-S4. Movies S10-S11 correspond to Figure 9E.
- Movie12 - Movies S12-S13. Micropipette Chemotaxis Assay of ACA-YFP/ctxA-/B--cells (Movie S12) and cAR1-YFP/ctxA-/B--cells (Movie S13). Live images sequences were converted to movies and processed as described in Movies S5-S8. Movies S12-S13 correspond to Figures 10A and B).
- Movie13 - Movies S12-S13. Micropipette Chemotaxis Assay of ACA-YFP/ctxA-/B--cells (Movie S12) and cAR1-YFP/ctxA-/B--cells (Movie S13). Live images sequences were converted to movies and processed as described in Movies S5-S8. Movies S12-S13 correspond to Figures 10A and B).
- Movie14 - Movie S14. Chemotaxis Assay of YFP-ACA/ctxB--cells. YFP-ACA translocates to the rear of a migrating cell and releases ACA-containing vesicles and the secreted cAMP attracts a neighboring cell. Images were captured at 1 frame/10 s and are played back at 5 frames/s. Movie S14 corresponds to Figure 10D.