E(spl)-C bHLH gene alignment: >Teleopsis_M8 ------------------------------------------------MAKKQQYQRTKKPMLERHRRARVTNCLSTLRQLIAECS---DNDNVLRMDKIVMLETTIAYMRQQQSAKKNK------------QQRTAAADIMHNFRHGYMNAVEE-VSRMLASIPGVNIEMGKTIMTHLGRVYNRLQVK--------------------QQKIQQQQQRSYYRNQLPIMNL----------------PQAALSPASSGYYSDDCESTK----------------------------STKSPQVETIPQENIWRPW >Haematobia_M5 ----------------------------------------------------------------------MNKCLDALKELVAN---SQGSDEILRMDKAEMLESALEFMKTQVKNQQIA------------VKLPAM-VPIDSFRNGYMNAVNE-VSRVMAATPGININTGKAVMTHLGAEFNRLLQQT-------------------TVPVVHDSATTSASTSAV--------------------IKAPLSPASFGYCSDWEDHQS--------------------------------SQGFPASHQFMGGPW >Haematobia_M8 ---------------------------------------------MEYATKTEIYQRIKKPLLERQRRARINKCLDGLKQLMID---LNGDSKILRMDKAEMLETTIGFLRTYNNKNK------------VITTKINK-YPWMPSANGYMNAVNE-VSRVLASTPGMTVEMGNFVMSHLGRSYKRLQQQY-------------------EVNYQQGYNTATPQRRASMQPLTV--------------ECCPLSPASSGYHSDDNLSPA------------------------------NSPIAAQQKQQGLWKPW >Cochliomyia_M5 -------------------------------------------------------------MLERQRRARMNKCLDQLKELVAE---FQGNDAILRMDKVEMLECALALVKKEVKKSQ----------------VPAV-IPMDSFRHGYMNAVNE-VSCVMASTPGMNVQLGKSVMIHLGGEYNRLLQLQN------------------SVPQQEQQQQPQQVS-----------------------CSAPMSPASSGYHSDECDEDS------------------------------SMSSPVVAASAPIWRPW >Ceratitis_M5 -----------------------MSPNTKSVQQQQ----------QFYATKTELYLKVKKPLLERQRRARINKCLDTLKTLVAE---FQGDDAILRMDKAEMLESTIAFMRQHSRRQRTT----------MPAVAPLA--PMDGFRNGYMNAVNE-VSRVMAAMPGMSVQVGKSVMTHLGSEFNRLLQQQ-------------------HQQQQQQQQQQQTIVERVEEKVIEK-------------VSRPSSRASSGYHSDCEHDXS-----------------------------DASPAPCSSAARVXSSTW >Glossina_M8 ---------------------------------------------MAYSTKTEIYQKIKKPLLERQRRARINKCLDSIKRLVVE---LQGDENILRMDKAEMLESTIIFLRSRKIKPT--------------KCSSHV-APVNTFRNGYINAVNE-VSRLLASTPGVSVDMGKSVMTHLGRSYNRLQQEY-------------------HHQQKEMLT-----------------------------IQCPHSPASSGYHSDNTQSPP----------------------------------ATPTPAFKLWRPW >Dmel_M8 ---------------------------------------------MEYTTKTQIYQKVKKPMLERQRRARMNKCLDNLKTLVAE---LRGDDGILRMDKAEMLESAVIFMRQQKTPKKVA------------QEEQSL--PLDSFKNGYMNAVNE-VSRVMASTPGMSVDLGKSVMTHLGRVYKNLQQFH-------------------EAQSAADFIQNSMDCSSM--------------------DKAPLSPASSGYHSDCDSPAP----------------------------------SPQPMQQPLWRPW >Dpse_M8 ---------------------------------------------MEYTTKTQIYQKVKKPLLERQRRARINKCLDTLKTLVAE---LRGDDGILRMDKAEMLESAVIFMRQQKSNKTSAS---------TATPAQSA-LPLDSFRNGYMNAVNE-VSRVMASTPGMSVDLGKSVMTHLGRIYKNLQQFH-------------------EAQTTMAVESESEMDCSSSS------------------SAAPLSPASSGYHSDCESPAP---------------------------------SPMPEESEKLWRPW >Dvir_M8 ---------------------------------------------MEYTSKTQIYQKVKKPLLERQRRARINKCLDTLKTLVAE---LRGDDGILRMDKAEMLESAVVFMRQQKMGKSET----------ATPASPAM--PLESFRNGYMNAVNE-VSRVMASTPGMSVDLGKSVMTHLGRVYKNLQQFH-------------------EAQTMSESEATTELSINC--------------------SSAPLSPASSGYHSDCESPAP---------------------------------SPMPVEGEKLWRPW >Dmel_M5 ---------------------------MAPQSNNS----------TTFVSKTQHYLKVKKPLLERQRRARMNKCLDTLKTLVAE---FQGDDAILRMDKAEMLEAALVFMRKQVVKQQAP-------------VSPL---PMDSFKNGYMNAVSE-ISRVMACTPAMSVDVGKTVMTHLGVEFQRMLQAD-------------------QVQTSVTTS-----------------------------TPRPLSPASSGYHSDNEDSQS--------------------------------AASPKPVEETMWRPW >Dpse_M5 --------------------------MAPQANSTE----------TAFVSKTQHYLKVKKPLLERQRRARMNKCLDTLKTLVAE---FQGDDAILRLDKAEMLEAALVFMRKQVVKQQAP-------------VSPL---PMNSFKNGYMNAVSE-ISRVMACTPAMSVDIGKTVMTHLGVEFQRM-----------------------LQPTQLES------------------------------SSRPASPASSGYHSDAEESEA----------------------------------AASPKPDTMWRPW >Dvir_M5 -----------------------------MAPQTE----------TAYVSKTQHYLKVKKPLLERQRRARMNKCLDTLKTLVAE---FQGDDAILRLDKAEMLEAALVFMRKQLIKQQAP-------------VSPV---PMDSFKNGYMNAVSE-ISRVMACTPAMSVDVGKTVMTHLGIEFQRMLQAD-------------------QQPEQHQQQQTELESMP---------------------ISRSASPASSGYHSDAEDSEA--------------------------------AVSPQSGSHSMWRPW >Teleopsis_M5a -------------------------------MCAK----------VEYISKTQHYLKVKKPLLERQRRARMNKCLDALKTLVAE---LQSDDAILRMDKAEMLETTIVYMRSQAVCKLKT-------------AWTSP--PMDSFCNGYMNAVNE-VSRVMAATPGMSVELGKSVMSHLGVEFNRLLQHK-------------------TQQQHQDTLSTKSH------------------------FPRPVSPASSGYHSDCDESSM-----------------------------------ATPVLSTVWRPW >Teleopsis_M5b -------------------------------MSSK----------VVYRSKTEHYLKVKKPLIERQRRARMNKCLDTLKTLVAE---FQADDAILRMDKAEMLETAITFMRSQTIQKPQN-------------VTTTP--SMDGYCNGYMNAVNE-VSRFMAATPGMNVQIGKTVMTQLGFEFKRLQQN--------------------KSQLTAVC------------------------------SSRPVSPASSGYHSDCDESSM-----------------------------------STPVEKPIWRPW >Dvir_Md ------------------------------MAVEG----------QRFMTKTQHYRKVTKPLLERKRRARMNLYLDELKDLIVDTM-DAQGEQVNKLEKADILELTVNYLKTQQQQRLACGSAGTPPDMSTPTSPPANQLNFDKFRAGYTQAAYE-VSHIFSTVPGVDLKFGTHLMKQLGHQLKDMKQQL-------------------ISPVP---------------------------------SEDAIEPVNLANGKQQRSTSP---------------------------------QENLDKGEDVWRPW >Dmel_Md ------------------------------MAVQG----------QRFMTKTQHYRKVTKPLLERKRRARMNLYLDELKDLIVDTM-DAQGEQVSKLEKADILELTVNYLKAQQQQRVAN----------PQSPPPDQ-VNLDKFRAGYTQAAYE-VSHIFSTVPGLDLKFGTHLMKQLGHQLKDMKQEE---------------------------------------------------------EIIDMAEEPVNLADQKRSKSP--------------------------------REEDIHHGEEVWRPW >Dpse_Md ------------------------------MAVQG----------QRFMTKTQHYRKVTKPLLERKRRARMNLYLDELKDLIVDTM-DAQGDQVSKLEKADILELTVNYLKTQQQQRLAS-----PSSPSSTSSSASSQVNFDKFRAGYTQAAYE-VSHIFSTVPGLDLKFGTHLMKQLGHQLKDMKEEL-------------------DTPDLSE-------------------------------EPVNLSDRKSEEHHHEERPTS------------------------------PPEDNDNPNGEDVWRPW >Glossina_Md ------------------------------MAVDG----------QKLMTRTQHYRKVTKPLLERKRRARMNFYLDELKELIIDTL-EAEGEHINKLEKADILEMTVNYLKAQRNNKTDFKH--------TITDGTCLVVDREKFREGYTQAAYE-VSKVFNTVPDLNVKFGTRLMKHLGYQLKDLKNCH---------------------------------------------------------NSKQLDNNKPQALENEANPFK---------------------------------------ESYVWRPW >Teleopsis_Md ------------------------------MSVDG----------QRFMTKTQHYRKVTKPLLERKRRARMNLYLDELKDLIVDTM-EAQGEHVTKLEKADILEMTVNYLKTHKHEVARTIRKDTD----IVSGNSVN---YEKFRAGYTQAAVE-VSKIFNTLPGLDVKFGTRLMKHLGYQLKDIQPSI---------------------KKCNETKEVEFIKP----------------------KDYPVRRKSAENIQHGLKIQN----------------------------------LQAKPGEDVWRPW >Haematobia_M3 ----------------------------------------------MEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECL-TQEGEHVTRLEKADILELTVEHMRKLKQRGNLA------------VRSSTLNAHVESFRSGYVHAADQ-ISQVLLQ-QYFDDDVRSKLMKFMSTRLLDMQHNLGLQNTMGGPIVPEASACTSTPPTAMHTSDHTRTLMDET-------------------YLAHLHRANSSHNRIIDSKDS-----------NDMIDIITVDNSMDSSASSALSVSSSNSDNSVWRPW >Cochliomyia_M3 ----------------------------------M----------VMEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECL-TQEGEHVTRLEKADILELTVEHMRKLKQRGNLA------------LRTTTLNAHVESFRSGYVHAADQ-ISQVLLQ-QYIDDEVKGKLMKFLSSRLMDMQHNVL-------IHSST------NTSASSPAMQKTFFDNVIFREQTKLATVTTTPQPPPLPLPTPTTTTSPQYDSASNATKIMNNNNNSDDLKENDMIDIITVDNCNSMDTNSVLSNDSNNSETVWRPW >Teleopsis_M3 ----------------------------------M----------VMEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECL-QQEGEHVTRLEKADILELTVEHMRKLKQRGSLS------------LQTPNSNAHVESFRSGYVHAADQ-ISQVLVQQRQTD-EIGGKIMKFLSSRLIEMQHQLLRSTMILPQQIPSVNTTNNGEPNLPATTPNLFAEFKSDMFKSEQIK-----------YQHMIYKNNYDFINVSANESQ-----------------------GATDNSSDISVDDLSNADTVWRPW >Dvir_M3 ----------------------------------M----------VMEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECL-QQEGEHVTRLEKADILELTVDHMRKLKQRGGLSLQAVGTAGGGNVAAPSTSAAHVESFRSGYVHAADQ-ITQVLLQTQQTD-EIGRKIMKFLSTRLIELQSQL-------------------LQQQQQQQPPAVGYA-----------------------SGRLAFPLLGGYGAAAAASYS------AFLAGKDELIDVTSVDGTALSETASVSSQESGASEPVWRPW >DmelM3 ----------------------------------M----------VMEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECL-QQEGEHVTRLEKADILELTVDHMRKLKQRGGLSLQGVVAGVGSPPTSTSTA--HVESFRSGYVHAADQ-ITQVLLQTQQTD-EIGRKIMKFLSTRLIELQTQL-------------------LQQQQQQQQHQQQQIPQS--------------------SGRLAFPLLGGYGPAAAAAAI---SYSSFLTSKDELIDVTSVDGNALSETASVSSQESGASEPVWRPW >Dpse_M3 ----------------------------------M----------VMEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECL-QQEGEHVTRLEKADILELTVDHMRKLKQRGGLSLQGPVAG---GSPPPATSTAHVESFRSGYVHAADQ-ITQVLLQTQQTD-EIGRKIMKFLSTRLIELQTQL-------------------LTQQQQQQQQSGFA------------------------SGRLAFPLLGGYGPAAAAAAV---SYSAYLSGKDELIDVTSVDGTAISETASVSSQESGASEPVWRPW >Glossina_M7 --------------------------------MIT----------VNEMSKTYQYRKVIKPQLERKRRARINKCLDDLKELLVECM-QQNGQSNVKLEKADILEVTVQHLRKLKSDPQQQ----------IACNNHIV--NKQSFRNGYIQAANE-VSHCLASLPKIDITFGTKLMTHLGLRLNQLESAE--------EMTPTSI----NTPLTLDCSITQFS------------------------RPILHSPVSSGYASDNDSLRS------------------------SPSFCPSAQGLLKVSSTSLWRPW >Ceratitis_M7 -------------------------------MATS----------AYELSKTYRYRKVMKPLLERKRRARINQCLDELKDLMAECVQQQSGDAEVKLEKADILELTVEHLRKMRQHQKKA-------------KHIDD-DVRQSFRSGYIKAAHE-ISRCLATLPNVDIQLGTHLMTQLGLRLNQLESQE--------QQVRTAV----NQPLSVHCGPATTSSS----------------------RASMYSPVSSGYASDSESTAS---------------------------------------SQS----- >Teleopsis_M7 --------------------------------MAT----------KYEESKTYQYRKVMKPLLERKRRARINKCLDDLKDLIGECMQQQCGDPSVKLEKADILELTVQHLRKLKTQKQNS-----------TQDNTKS---QHSFRSGYIQAANE-VSRCLASLPKVDVAFGTQLMTHLGLRLNQLESAE-------EAHTAI------NTPLSINCGGASAHIA----------------------STAMYSPVSSGYASDSENSRC-------------------SPALSSKSFSSICXSRALQINKQVWRPW >Dpse_M7 --------------------------------MAT----------KYEVSKTYQYRKVMKPLLERRRRARINMCLDELKDLMVQCT-VQSGDG--KFDRADILEVTVDHLRKLKQARIEATA--------AAAKATTNTTPEQSFRDGFIRAADE-VSRALASLPNVDVVFGTHLMTHLGLRLNQLETPT-------------------AAPKP---------------------------------MNAPLSI------------------------------------------------------------- >Dmel_M7 --------------------------------MAT----------KYEMSKTYQYRKVMKPLLERKRRARINKCLDELKDLMAECV-AQTGDA--KFEKADILEVTVQHLRKLKESKKHV---------------PAN--PEQSFRAGYIRAANE-VSRALASLPRVDVAFGTTLMTHLGMRLNQLEQPM---------EQPQAV----NTPLSIVCGSSSSSSTYSSAS-----------------SCSSISPVSSGYASDNESLLQ------------------------------------ISSPGQVWRPW >Dvir_M7 --------------------------------MAT----------KYELSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMAECV-AQTSDA--KFEKADILEVTVQYLRKLKQTKSAN-------------ATASA--TEHSFRAGYIRAANE-VSRALAALPKVDVAFGTTLMTHLGMRLNQLEQPV-------EQPSPMAP----HAPLSINCGSGYSTSS----------------------SRDAYSPVSSGYASDSDCSSG------------------------SSTPIAAQSLLQISNTGSVWRPW >Ceratitis_Mg -------------------------------MSIQ----------VSEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVVTL-ESEGEHVTRLEKADILELTVAHLQKLKQQRKAA----------ASKGGAMS--QAEGFRSGYIHAVNE-VSRSLSQLPDVNVNLGTQLMTHLGQRLNQLQPAV-------QPPTPI------NAPLSVHITHNTARAH----------------------YSVPISPISSYNGSPNSSTSS-----EKHSSSCGSQIFLPPYSDHSPIDARLATNVAPS?EEDVWRP- >Glossina_Mg -------------------------------MSTE----------ISEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVSHLQRMKQHRKLN------------GNNSSSMTQAEGFRSGYIHAVNE-VSRSLSELPGVNVNIGTKLMTHLGQRLNQLQPPV-------KPSLPV------TSPLSVHIATTCQRKQ----------------------YSAPISPLSGYASSPDSSLCS---------TKRFTPLLTTCPSTPIDVTSVDANDAEEEEEENV---- >Dpedo_Mg ------------------------------MSSSQ----------ISEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVTHLQKMKEKRQHK----------RATGDESL-SPAEGFRSGYIHAVNE-VSRSLSHLPGMNVSLGTQLMTHLGQRLNQLQPAV-------KEALPI------TAPLSVHIASRDA-------------------------YSVPISPVSSFAGSPNSSLAS----------SERLGSASLLTTCSSIDVTKMELEVDSEDEENVWRPW >Dmel_Mg ------------------------------MSSLQ----------MSEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVTHLQKMKQQRQHK----------RASGDESL-TPAEGFRSGYIHAVNE-VSRSLSQLPGMNVSLGTQLMTHLGQRLNQIQPAE-------KEVLPV------TAPLSVHIANRDA-------------------------YSVPISPISSYAGSPNSNTSS-------------------TSHSLLTTIDVTKMEDDSEDEENVWRPW >Dvir_Mg ------------------------------MSSIQ----------ISEMSKTYQYRKVMKPLLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVTHLQKMKQQRQHKR---------ANGGAETL-TPAEGFRSGYIHAVNE-VSRSLSQLPGMNVSLGTQLMTHLGQRLNQLQPAV-------KEVLPV------TQPLSVHIASRDA-------------------------YSVPISPISSYAGSPNSSVAS--------EQLGSASLLTTCSSNLSIDVTKMEEVADSEDEENVWRPW >Teleopsis_Mg ------------------------------MAAAQ----------ISEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVTHLQKLRQQRKQA----------AAKGNTTM-SQAEGFRSGYIHAVNE-VSRSLSELPGVNVNLGTQLMTHLGQRLNQLQPAV-------KPPVPV------TSPLSVHIGNVNFRGH----------------------YSVPISPISSYTGSPNSSISS-----------EKHTPLLVTCPNSPIDVTTVDINIDVEEEDNVWRPW >Haematobia_Mg -------------------------------MSLQ----------MSEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVTHLQKMKQHRLAKA---------SNGSSNTM-SQSEGFRSGYIHAVNE-VSRSLSELPGVNVSLGTQLMTHLGQRLNQLQPPV-------KPSLPL------STPLSVQIAAPTFKGQH---------------------YTVPISPVSSYSSSPNSSVGS-----------EKHATSLLTTCQSTPSIDVTSIDVDEEDEENVWRPW >Cochliomyia_Mg -------------------------------MSVQ----------MSEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATL-ESEGEHVTRLEKADILELTVSHLQKLKQQRKLN----------NSNGNTTM-NQAEGFRSGYIHAVNE-VSKSLSELPGVNVSLGTQLMTHLGQRLNQLQPPV-------KAALPV------TSPLTVQIGAPTFRGQ----------------------YSVPISPVSSYSTSPNSSIGS----------EKQASSQLLTTCPPTPCIDVTSIDVEAEDEENVWRPW >Cochliomyia_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRL---------NGNADSKL-TIAESFRAGYIHAANE-VSKTLATVPGVSVDLGTQLMSHLGHRLNYLQVIV--------PTA--------VTPLVAPTASTTHFSAMPAQTHLGSTSLCSSP------EPNLITPPPSECGSSDSGSCS----------------------------------PTPSETAAMWRPW >Glossina_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRLT--------ASSQDTKL-TIAESFRAGYIHAANE-VSKTLASVPGVSVDLGTQLMTHLGHRLNYLQVIV--------PTT--------VPPLGITVSQQTTPTQLGVIVNQTQLASNYFAANSGN-DAVLITPPPSECGSSEHGGCS----------------------------------PAPSDSGSMWRPW >Ceratitis_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRLGGVGAGT--GAVQDPKL-TIAESFRAGYVHAANE-VSKTLAALPGVNVDLGTQLMSQLGHRLNYLQVVV--------PSVPL------XGGMSQPQPFMPTTVM----------------------PSTTVTAAAHQYYSLSGSERS----------------------------ASSSPIVITPPPSEC---- >Teleopsis_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRLGGV------PNGQDTKA-TIAESFRAGYIHAANE-VSKTLAAVPGVSVDLGTQLMSHLGHRLNYLQVVV--------PTIPI------GVPTANVTTAHQQQQQQQTQLLQGQYFCITPEQR----SPVIITPPPSECGSSESGSCS----------------------------------PAPSETGNMWRPW >Dvir_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRLGSSSNA---SVAGDSKL-SIAESFRAGYVHAANE-VSKTLAAVPGVSVDLGTQLMSHLGHRLNYLQVVV--------PTLPIGV----TVPLQPNDELSSNSSSSSSP------------------SPALVTPPPSECGSASGSCSP----------------------------APSEASTSSNSTGPMWRPW >Dpse_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRLSSSVSPS--ASSADSKL-SIAESFRAGYVHAANE-VSKTLAAVPGVTVDLGTQLMSHLGHRLNYLQVVV--------PTLPIGM----GVPLQTPVSGVD--------------------------DAALVTPPPSECGSLESGTCS------------------------------PAPSEASSTSGPMWRPW >Dmel_Mb --------------------------------MVL----------EMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECL-TQEGEHITRLEKADILELTVEHMKKLRAQKQLRLSSVTGGV--SPSADPKL-SIAESFRAGYVHAANE-VSKTLAAVPGVSVDLGTQLMSHLGHRLNYLQVVV--------PSLPI------GVPLQAPVE-----------------------------DQAMVTPPPSECDSLESGACS------------------------------PAPSEASSTSGPMWRPW >Rhynchosciara ------------------------------MAYTQESVIVKGTAPLEPVSRTYQYRKVMKPLLERKRRARINRCLDELKELMVTAL-QSEGENVSKLEKADILELTVRHLRNLRCHQRSS-------------ANRFA--DSDRFRAGFEHAAXRGISLSRIHTRALTFRLGTK------SKWNILPPTV----------------------------------------------------------------------------------------------------------------------------- >Lutzomyia ----------------------------------M----------AEPVSRTYQYRKVMKPMLERKRRARINRCLDELKELMTGAL-QTEGENVAKLEKADILELTVRHLHTLRRQNALS-------------VRPEH-TYADRFKAGFRHCAAE-VTTFLS---GMDQATRMHLTSHLSGCIRRLDVTQ--------PPVWVG-----PTPASPPRPAPPNYANIQTPP-----------------PVMPLQPSSPPAHQGDESSGP-------------------------------------------WRPW >Phlebotomus MKFEMPFVANGPNSEEYQTVIVKQG----PSPPPQ----------PEPVSRTYQYRKVMKPMLERKRRARINRCLDELKELMVTAL-QSEGENVSKLEKADILELTVRHLHKLRRQQRLC-------------ANPVI--DSDRFRAGFTHAANE-VSRCLASTPGVDITLGTKLMTHLGHRLNELDKIQ--------PLSIHVS----QQPYTPPSSPSTSICSSEAGPH----------------YTMPLTPASSRSCRSPSPRSE-------------------TLLRPTRQGSYGLYRLFKLQPGGVWRPW >Aedes --------------MVYANMVAEPTMGGKIPQPGS----------LEPLSRTYQYRKVMKPMLERKRRARINRCLDELKELMVSAL-QSEGENVSKLEKADILELTVRHLHKLRRQQRLT-------------VNPVI--DADRFRAGFTHAANE-VSRCLASTQGVDIKLGTKLMTHLGHRLNDLDKVS--------PLSVHVEQNLQSPPISPFSTSSSTAADLQS-------------------YQMPLTPQSYRSGSGNSNSPS------------------PNPSECSSTGSDQQTSGLLKIQESVWRPW >Anopheles --------------------MVSESAGINGKLQAS----------LEPLSRTYQYRKVMKPMLERKRRARINRCLDELKELMVSAL-QSEGENVAKLEKADILELTVRHLHKLRRQQRLA-------------ANPVL--DADRFRAGFTHAANE-VSRCLASTPGVDIKLGTKLMTHLGHRLNDLDKVS--------PLSV-------HMPLTPQSYRSEESGLLA--------------------PSTTPSPNPSECDDHHHHHTG-------------------------------GLLKIQQSGESVWRPW >Bombyx ----------------------MSYEIAYSVAEDG----------PQPVSRTYQYRKVMKPMLERKRRARINRCLDELKELMVSAL-QSEGENVAKLEKADILELTVRHLHKLRRQRRLS-------------LNPTV--DVDRFRAGFTHAANE-VSRCLASIPGVDVRLGTQLMTHLGHRLNEMQRAAAGTE---------------TPPASPPSPALSTVSSSSGYVS----------------PSPPASPMPLQTATPLDCSMN----------------------------------TSLPKASAVWRPW >Polypedilum -----------------MKNMCYETSYQNIVSEVNVMKSKSSMMTSEPVSRTYQYRKVMKPMLERKRRARINRCLDELKELMVSAL-QAEGENVSKLEKADILEMTVSHLHKLRRQQRLA------------HANPVI--NEDRFRAGFTHAAN----------------------------------------------------------------------------------------------------------------------------------------------------------------- E(spl)-C Brd gene alignment: >Glossina_M2 ------MFTDTKNVINACHYNDVTMH------------------------------------------TMMMVEKSEKCTLKKILKPLERLISL--KTRNKKATVTSSVPNNVNLNNALANVNKESTYDKPVKQAKEISTNHKLLTDEHWMAVDGDGDGDPSKHNEIILRLQPEVFIRSVNIEKPSSLNACIHCVYGRNCQHVVSNSSNICDENN--CL-------------------------ALPVSTEAIENSPF------------------------------------------------ >Ceratitis_M2 ------MSLTGVNTHL----------------------------------------------------PPSTPMEKSKRKLCKMWRPLLRLMAR--KRHNSATTTAIRSSNVTRLHPNNVHLN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- >Teleopsis_M2 ------MFTDTKNLSNATLLDSNLF-------------------------------------------NSSEKSK---GKLRRVWKPLLRLMSMKTKTKSSTGTSTKTHPNNINLNNATSVKDIYELSTALLEDYTAMIRKVAPEVEQESIPALPVTDTKHTTTVVSSASIEKTPTP------------NCTGCIYGRNCQHEQFHQDYLFNWTADVYD----VDTRNV----AAELVNTTSTTGLPVQYISTDDGTFFWTTSEHRVDD--------NLLHAWLCQTLKQLPVQTALL-------- >Dvir_M2 ------MYLDTKNLTAGCSNNNTTA-------------------------------------------TTASTAPKTGRRMRKVWQPLRRLLTV--GGKGRPA--SQPHPNNVDLNNTQMLPPLELLHTD--DNDSKATPDESINKFAELRLQQVHEELTQTPPPPNTPTLISSVYVEKASTQ------SCNACQHGRNCQHTHHTTTSSNSSSN--NNSHNLTENVAAQSTPTPTSTPTLWDLQLPLQYISTDNGTFFWANTQERVDD--------DLLHAWLCQSFSQLPGTLC---------- >Dmel_M2 ------MYLDTKNLTAS---STSALT------------------------------------------AATASNSKSTRRMRNVWKPLSRLLKVGGKGRTAQA--TLAHPNNVDLNNTQQQQQQQQQELLI-EDDTKSTPEESIHKYGLELRQLPEEEQVSLTQPPNTPTLISSVHVDKVSAQ------SCGNCQHGRNCQHRHHSSSNINSSSS--SS----NMNSSS----ATQLPSNLWDLQLPLQYISTDNGTFFWANTQDRVDD--------DLLHALLCQSFSQLPGTLC---------- >Dpse_M2 ------MYLDTKNLTASSTNTNTNSSSSSIHTSASMTATA----------------------------ATPLGVPKTVRRMRKVWQPLRRLLTV--GGKGRPAPLTLAHPNNVDLNNTQQLQQQQQQIELILEDESKSTPEESIYKYGAELRQLPVEDEVSLTQPPNTPTLISSVYVEKASAQ------SCGACPHGRNCQHTHHNSNRNTTTAT--SS----ASATAT----SAQLPSNLWDLQLPLQYISTDNGTFFWANTQERVDD--------DLLHALLCQSFSQLPGTLC---------- >Dmel_BobA ------MFTETALVSNF---NGV---------------------------------------------TEKKSLTGASTNLKKLLKTI--------KKVFKNS--------------------------------------------------------------------------------------KPSKEIPIPNIIYSCNTEEEHQNWLN--EQ----LEAMA-----------------IHLH--------------------------------------------------------- >Dvir_BobA ------MIGENIQHG-----NTVEF-------------------------------------------AEKRSSTGAVVNLKKLLRTVTRI-----FNLSKPK--------------------------------------------------------------------------------------NMATDETGIK-SSAFATEEELQNWLN--EQ----LELKE-----------------MSLR--------------------------------------------------------- >Dvir_Brd ------MVYESLLNATN---SALYV-------------------------------------------DVRKRSLKPLRSIQNVFKV-----------------------------------------------------------------------------------------------------------------ACKAFQWKKQNNATD--MD----IKELE---------------LIKPCNVA------------------------------------------------------- >Dmel_Brd ------MAYETLMNTTIYTTTPVCQ-------------------------------------------KKTYTPA---KAMKKIFKVA--------CKIFKAS-------GHQQLKNHS----------------------------------------------------------------------MPKELPLPTSEEELQNSQNEQLEAEL--VQ----L---------------------------------------------------------------------------------- >Dpse_Brd ------MAYETLLNSTY---SPVYV-------------------------------------------KKTYTPS---KAVKKIFKMA--------CKLLKAS--STTSKGHRELKNTS----------------------------------------------------------------------PPAHEENSSQIECLVLSEEELQNSQN--EE----LEAAA------------------TAQLLQL----------------------------------------------------- >Teleopsis_M6 -----MICLKNSK-------------------------------------------------------TSLKSFGKFKVSARNLLKKLF-------VQKNKYN--SKNINNNNTKQKNN---------------------------NVAAITTSIEIAQNEANDR------------------------LMKARASAGLEAAVVNGVDDVVDAYQ--VP---------------------------RIMCVETSDGCFYWDNNSLQKLP---------ADIDLQHLTYRRHSFTTTRQFH------ >Dvir_M6 -----------------------------------------------------------------------------MSKVKNLIAKML-------QRRGKCN--VSGSSSSGHSNNN-----------------------------------------------------------------------NISHCQHYESLEEIAQNQANERMLQE--TA---MGTHQL-------------------LFCLETADGSFYWHAQ------------------------------------------- >Dmel_M6 -----------------------------------------------------------------------------MSKVKNLLAKML-------QRFGKNS-------------------------------------------------SQADSQRYDSLEE------------------------IAQNQANERMLRATQVGLEEHL------------------------------------VICLETEAGSFYWHSQ------------------------------------------- >Dpse_M6 -----------------------------------------------------------------------------MSKVKNLLAKIF-------QRFGRGH--TNCHGGSNDSQRYD----------------------------------------------------------------------SLEEIAQNQANERMLMETLIRCEEEH--L-----------------------------VICLETAAGSFYWHSQ------------------------------------------- >Glossina_M4 ------MCKEISSNTAY---AMMGLK------------------------------------------NNEKKLT---YSLKKLIKQYF-------KQRKSDK--LQHFENAGETLESL------------------------------NSIESLENNQNADIES------------------------ESENMVNECRSEVFSAHNLEDYELSD--VL-------------------------PIPVHFMRTDQGTFFWTTATDIAAD-------NDLAEPLYCCT---TNQVAVNQMQDRWAQA >Dmel_M4 ------MCQNKINTNNT---------------------------------------------------MTIKSNKKLSYSVKKLLQKIF-------KQQQRVE-------EEQNLKNALK-------------------------ANSLESLESMENSRNADLES------------------------ASICASLESCENEANERLSQSCEIED--YD----FEQLP----------------TVPVHFVRTAHGTFFWTAVSDLPAD-------NDLVEPLYCST-----SNAIAIPQDRWVQA >Dpse_M4 ------MCQNKTNDSNT---------------------------------------------------MSIKSNKKLSYSVKKLLQKIF-------KQQQQQL-----VEEEQNLKNTLK-------------------------ANSLESLESIENSRNADLES------------------------ASVCASMESYENEANERLSASCDMED--YE----LEQLP----------------SVPVHFVRTAHGTFFWTAASDLPAD-------NDLIEPLYCST---ANEIAVSQFQDRWVQA >Dvir_M4 ------MCQSQNIVN-----------------------------------------------------NMNIKSNKKSYSVKKLLQKIF-------KQHQQQVEEQQNMQNTHKANSLE-------------------------------SLESIENSRNADLESIAN---------------------KSCAASLESYENEANERLSASCEMED--YE----LEQMP----------------TVPVHFVRTAHGTFFWTAASDLPAD-------NDLVEPLYCST---ANEIAVAQFQDRWVQA >Teleopsis_M4 ------MCVEIKNNNMN---AIIELDV-----------------------------------------KQTKKVS---YSIKKVLKQLF-------KQHKTEK--LITKQNIENTKKES----------------------------SMESLESIQNSQNADIESGCV---------------------SLESYQNDVNERLSACTEPVSYEMED--YE----YGDIT----------------TVPVHFARTAHGTFFWTAASDLPVD-------NDLIEPLYCCT---ANQIAVPQMQDRWAQA >Ceratitis_M4 ------MYTVEYENNMNMS-------------------------------------------------AEQKQQKKASYSIKRLLKQLF-------KQHNKSQ--AQKQQQQQALIENFENTKK---------------------ANSLESLESLENSRNADLES------------------------EAECISLESTENDANERLAASCELED--YE----FADIT----------------TVPVHFLRTAHGTFFWTANSDMPAD-------NDLIEPLYCST---GNQIATSQMQDRWAQA >Haematobia_M4 ------MCVEISKESFN---VTNVTNL-----------------------------------------SNKKTMT---YSIKRLFKQLF-------KHQKCQK--LEIIDKNQTSESVE--------------------------------TLDNNNNVNTTLES----KL----------FDI-SQSTNILENSCEK--DFGYLSDQQSFEMDP--FESLI-EEKSYE----LEDFDFSYVSTTVPVHFMRTDNGTFFWTANTEIPAD-------NDLPQPMSC-----STNNQIACRQNRWTQV >Haematobia_Tom ------MSFMTREYKFN---TTIQM-------------------------------------------NKKDQHTMAMRSLKKLVKPLLRLVRK--KQLMRKT--LAEIQQNTYNSSLED------------MR-----------KDPVSLHATQDNVANEALEQ----RL----------YS------EIRQCPAN---AAVIVHQGHQQHVVS--VN----QQQNY-----------------IPVHFARTDSGTFFWTSMDIAQQQ-----------------------EMKMRCQLDRWAQA >Dvir_Tom ------MSFIMREYKFE---TSTTMQ------------------------------------------RKSDQHTMAMLSLKKLVKPLLRLIKK--KQLIRKT--LAEVQSQNEINSSL--------------E-----------DMRKDSVASCDNMANELLEQ----RL----------FN------DLRECPNN---AAIIVQQDDQQSVVP--VQ----PDQTF-----------------IPVHFARTTSGTFFWTSADGTCLQ---------------------QQEQQMRERFDRWVQA >Dmel_Tom ------MSFITREYKFE---SSKVME------------------------------------------PKSDQHTMAMRSLRKLVKPLLRLVKK--KQLLRKT--LAEIQNQNAVNASL--------------E-----------DMRKDPAASCDNMANEELEQ----RL----------YT------DLRQCPTN---VAMIVQQGQQQSIVP--VH----PEQTF-----------------IPVHFARTTSGTFFWTSAEGARQH----------------------HEQQLRQQFDRWVQA >Dpse_Tom ------MSFITREYKFE---SSMVQ-------------------------------------------QKSDQHTMAMRSLKKLVKPLLRLVKK--KQLLRKT--LAEIQNQNDINASL--------------E-----------DMRKDPAVSCDNMANEQLEQ----RL----------YN------DLKQCPSN---VAMIVQQGQQQHVVP--VQ----PEQTF-----------------IPVHFARTSSGTFFWTTAEGARQH----------------------QEQQLRQQFDRWVQA >Ceratitis_Tom ------MPFLTREYNFH---ESELA-------------------------------------------AAKDQRTMALRSVKKLLRPLMRIIKK--KQLLQKT--LAEVKQQNDYNSSLED-----------MR-----------KDPAALNAYDDNLANEALEQ----RL----------YE------ELQQSAPE---AAIVVRQGDSQQVVP--VQ-----QQTY-----------------IPVHFARTANGTFFWTSSDMPRQQ-----------------------ELQLRQQLDRWGQA >Teleopsis_Tom ------MLFITREYKFQ---NSVAV-------------------------------------------EKIDQHTTAMRSLKKLIKPLLRIIKK--KQLLKKT--LAEVQQQNAYNSSLEDMR----------------------KDPVAANAINDNLANEALEQ--------------LIYD------EIRHCGDN---AAVIVQQGQDQHVLP--VD----PKQEF-----------------IPVHFARTSNGTFFWTSTDIQRQR---------------------QQELELRQHLDRWGQA >Polypedilum ------RIID-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EIKHCDENS--AIHVYNEENNCQLTP--VS----NEEVY-----------------VPVHFAKTEMGTFFWTTTQRYDDD---------LIEAKQCTSNRQLKQQQSHSF-DRWVQA >Anopholes ------LAVCNSNNSIN---ENRYN-------------------------------------------SEKLAKSSAVYRLKKILKPLVTLLKS--NKSNKSPAKSCHHAQVDQRRSII--------------------------SYIDDYDCDYDNSANERLEAELVA--------------------ELEQCHATD--AAILVLEEQQLRLQP--VL----PEELY-----------------VPVHFARTEAGTFFWTTMPAREEK-----------------------QYAQQAYADRWAQA >Dvir_Ocho --MSKISAKNNKQFISN---NKKKFTMYSY--------------------------------------KSKVSPT---RRLTNILKPLL-------GHFFKLK--AKQPK-RSSYIASD---E---------KE-----------SEFDWLNNDMDNMSNEHLEN----RL----------IE------EIRQCAKEE--AIIVYNEDGSGELQT--FD----QGEYY-----------------VPVHFARTNGGTFFWTSLQPKSAK----------------------PYDIEWNFHDRWAQA >Dmel_Ocho ------MSAISKQKTLN---------------------------------------------------TMYKSKVSPSRRLKNLLKPLL-------GQFFNMK---KEQPKRSSYIASEE------------KE-----------AEFEWLSNDMDNMANEHLEH----RL----------IE------EIRQCAKED--AVIVYNENGSGELHT--ID----QEDYY-----------------VPVHFARTNAGTFFWTSVQPKACE----------------------PYAIEWNFLDRWAQA >Dpse_Ocho ------MSAITKQKNFN---NNNMY----------------------------------------------KSKVSPSRRLKNLLKPLL-------GHVFNLK--KEQPKRSSYIASAE-------------KE-----------AEFDWLNNDMDNMANEHLES----RL----------IE------EIRQCAKED--AIIVYNENGSGELQT--MD----QEDYY-----------------VPVHFARTNAGTFFWTSVQPKASE----------------------PYAIEWNFLDRWAQA >Haematobia_Ocho --MNTTNMNLSHQYNNN---NSVLYNNNNYKNQSYTIY------------------------------KSKVSPS---RRLKNMLKPVL-------GRIFKSK--AAKPK-RSSYIASE---E---------KE-----------SEFDWLNNDMENMANEHLEN----RL----------MD------ELRHSETGE--AIVVINEDGSRHLEY--VD----KENLY-----------------VPVHFARTNAGTFFWTSVQRENIE----------------------PYQIEWNFLDRWAQA >Teleopsis_Ocho ------------------------------------------------------------------------------------------------INQRKXA-------------------------------------------------------------------------------------------------AIIVYNEDGTRRLKT--FD----KEDFY-----------------VPVHFARTNAGTFFWTSIQRETIE----------------------PYQSNGIFXDRWAQA >Ceratitis_Ocho MSAAYATNMSSAQYDCN---NNNSCNSKKHNNSYYAATGTNESLLLQHSNIKAQQPNPLHQHWTDVQQKSKVSPS---RRLKNLLKPLL-------GRVFKSR--TKRQQNRNSYIAASPNGE---------RD-----------ADFEWLANEMDNRANEDLEQ----KL----------VE------EMHMCEDGA--AIVVYNEDGTHRLQT--IE----KDELY-----------------VPVHFARTNAGTFFWTSLQRETME----------------------PYKIQWNFMDRWAQA >Bombyx -----MSTYANNEYIIA---TNNSINENRYN-------------------------------------AEKMKNS----GIKALLRPLM--------KMIKKT--TKRVPAKAIDTCHE----------------------------------DAQNSSNELLER----KI----------YE------EMEACQSGA--AFLVYNQDETCDLVP--VN----KDTFY-----------------VPVHFARTEAGTFFWTAVSKPEAD----------FATAHCYTECQAAEQQHPVLQDRWVQA >Aedes -MHSHGYNVAHSNNSIN---ENKYN-------------------------------------------NMKLMKSSSVYKLKKILKPI--------AALLKSKKEAKRIKDTQEQFYYVP-------------------------EQNFVPDYDYDNDANERLEAQLLS--------------------EIAECHAN---AAIIVYEDNQVQIQP--VE----PEEQY-----------------VPVHFARTEAGTFFWTTMAREADS--------DLIQPCHCFSDHQVPQQE---FCDRWAQA >Teleopsis_Ma ------MCQQVQAKIN----------------------------------------------------SNNNNKMKPSYSIKKVLKTLF-------KKQQKKL------------------------------------------SNSLESLESLQNQRNY----------------------------EIEQHFYEEIVDNTVNEKLADLAVAE--------EQDIY-----------------VPVRFARTDAGTFFWTTNLLPVTSTIIPAADEDLLQPAFCYSNCQFPHMQ---FQDRWAQA >Dvir_Ma ------MCQQVAISNN----------------------------------------------------DNNNNKMKNSYSIKQVLKTLF-------KKQQKQQ--QQKHHRQNSLESLE----------------------------------SVDNLRNAEVEE------------------------VYYAEIDENAANEKLAQLVQEQELDT--------EEDIY-----------------VPVRFARTEAGTFFWTTNLQPVAS-----IDADLLQPAYCYSSQQHPFMQ---YQDRWAQA >Dmel_Ma ------MCQQVVVV------------------------------------------------------ANTNNKMKTSYSIKQVLKTLF-------KKQQKQQ-----QKPQGSLESLE----------------------------------SVDNLRNAQVEEAYYA--------------------EIDENAANEKLAQLAHSQEFEIVEEQ--ED----EEDVY-----------------VPVRFARTTAGTFFWTTNLQPVAS----------VEPAMCYS---------MQFQDRWAQA >Dpse_Ma ------MCQQVVVVPAA---------------------------------------------------NNSNNKMKTSYSIKQVLKTLF-------KKQQKQQ--QQQHPHRHSLESLE----------------------------------SNDNLRNAQVEEAYYA--------------------EIDENAANEKLAQMAHSQEIEMIEED--QE-----EDIY-----------------VPVRFARTQAGTFFWTTNLQPVAS----------VEPAFCYS---------MQFQDRWAQA >Glossina_Ma ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DIY-----------------VPVRFARTEAGTFFWTTNLQPVASTIVPSADKDLLQPAYCYSNYQFPLMQ---FQDRWAQA