Vertebrate ultraviolet visual pigments: Protonation of the retinylidene Schiff base and a counterion switch during photoactivation -- Data Supplement - HTML Page - 05206Fig7Legend.htslp -- Proceedings of the National Academy of Sciences

Fig. 7.
(A and B) Photochemistry of the E108Q mutant of the mouse SWS1 pigment at cryogenic temperature (75 K) on illumination at 305 nm. The dotted spectrum labeled PSS305 wild type in B corresponds to the photostationary state generated on illumination of the WT mouse SWS1 pigment under the same conditions. (C) The difference spectra between the photoproduct obtained after 15, 30, 45, and 60 min of illumination minus the dark state spectrum are shown and labeled 15 m, 30 m, 45 m, and 60 m, respectively. (D) Plot of the absorbance changes monitored at 400 and 280 nm during successive illuminations.