Fig. 8.

(A) Affinity purification from rat brain homogenate by using different NT fragments fused to GST. Samples were analyzed by SDS/PAGE and immunoblotting for clathrin, Eps15, AP-2, and hsc70. (B) Western blot of material affinity-purified from rat brain by using different parts of stonin 1. Samples were analyzed by immunoblotting for the m 2- and g -subunits of AP-2 and AP-1, respectively.