Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Supplemental materials and methods (construction of plasmids and gene replacements), strains and plasmids used in this study (Table S1), deduced functions of ORFs in fosmid 37F1 (Table S2), deduced functions of ORFs probably not involved in salinomycin biosynthesis in the sequenced region (Table S3), primers used in this study (Table S4), structures of polyether abtibiotics BL580Δ and mutalomycin (Fig. S1), gene organization of the fosmid 37F1 (Fig. S2), PCR products of polyether antibiotic producers with the degenerate primers Epo-110-F-2/Epo-1030-R-1 (Fig. S3), LC-MS analysis of salinomycin from S. albus XM211 and its derivatives (Fig. S4), LC-MS analysis of salinomycin from S. albus XM211 and its mutants (Fig. S5), multiple sequence alignments of epoxide hydrolases from various polyether gene clusters (Fig. S6), and phylogenetic analysis of epoxidases involved in polyether biosynthesis (Fig. S7).
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