Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Experimental procedures, characteristics of the used primers (Table S1), legends to Fig. S1 to S4, Movie S5, and Fig. S6 and S7.
PDF file, 29K. - Supplemental file 2 - Phylogeny of the selected strains, nearest relatives, and strains used for comparison of the MIC and biosorption capacities (Fig. S1).
TIF Image file, 7.5 MB. - Supplemental file 3 - TEM photographs of Bacillus cereus MM8, Lysinibacillus sp. HG17, Bacillus sp. CM111, K. rosea EP1, M. oxydans HG3, S. marcescens HG19, and Ochrobactrum sp. HG16 incubated in the presence of 20 μM HgCl2, 100 μM HgCl2, and 100 μM HgCl2 after an autoclaving step (Fig. S2).
TIF Image file, 7.8 MB. - Supplemental file 4 - Cartography showing the Mα/β and Lα signals of mercury overlapped on the TEM photographs of K. rosea EP1 after incubation in the presence of 100 μM HgCl2 of the live and killed bacterial biomass, respectively (Fig. S3).
TIF Image file, 7.7 MB. - Supplemental file 5 - Hg(0) volatilization assay on the negative control, positive control, and cultures of Microbacterium oxydans HG3, Ochrobactrum sp. HG16, Lysinibacillus sp. HG17, Serratia marcescens HG19, Kocuria rosea EP1, Bacillus sp. CM111, and Bacillus cereus MM8 (Fig. S4).
TIF Image file, 306K. - Supplemental file 6 - Tomography experiment, carried out between -60 and +60° with 1° increments, showing the extracellular localization of mercury beads for the strain Kocuria rosea EP1 (Movie S5).
AVI Video clip, 19 MB. - Supplemental file 7 - Scanning electron microscopy of Bacillus sp. CM111 showing the presence of a capsule (Fig. S6).
TIF Image file, 3.8 MB. - Supplemental file 8 - Characterization of the protein content of the EPS from Lysinibacillus sp. HG17 (Fig. S7).
TIF Image file, 7.9 MB.