Identification of Interaction Sites for Dimerization and Adapter Recruitment in Toll/Interleukin-1 Receptor (TIR) Domain of Toll-like Receptor 4

Supplemental Data

Files in this Data Supplement:

  • Supplemental figure 1 (.pdf, 415 KB) - Supplemental Figure 1: MAPPIT setup used in this article. In the pCLG MAPPIT bait receptor, the extracellular, transmembrane and JAK2 binding part of the leptin receptor are coupled to a 20x GGS linker (29) . This receptor is unable to induce JAK/STAT signaling due to the absence of a STAT3 binding site. The bait protein is attached to the 20x GGS linker. The prey protein is fused to several STAT3 recruitment sites of the gp130 chain. Interaction of bait and prey leads to STAT3 recruitment and activation. A STAT3 responsive luciferase reporter gene allows detection of the interaction.
  • Supplemental Table I (.pdf, 53 KB)
  • Supplemental Table II (.pdf, 108 KB)