Bone Morphogenetic Protein 4 Promotes Vascular Smooth Muscle Contractility by Activating MicroRNA-21 (miR-21), which Down-regulates Expression of Family of Dedicator of Cytokinesis (DOCK) Proteins

Supplemental Data

Files in this Data Supplement:

• Supplementary Table S1 (.pdf, 947 KB) - Previously validated miR-21 targets were enriched by bio-miR-21 pull-down.
• Supplementary Fig. S1 (.pdf, 726 KB) - siDOCK7 specifically downregulates DOCK7 protein level in PASMCs.
• Supplementary Fig. S2 (.pdf, 331 KB) - Rac1 inhibitor does not affect cytoskeletal remodeling in vSMC.
• Supplementary Information (.pdf, 241 KB) - Primer sequences for qRT-PCR and cloning
• Supplementary Fig. S3 (.pdf, 1.8 MB) - PDGF-BB downregulates miR-21 level.
• Supplementary Fig. S4 (.pdf, 626 KB) - BMP4 downeregulates endogenous mRNA levels of DOCK genes in vSMCs.
• Supplementary Table S2 (.pdf, 353 KB) - Housekeeping genes are not enriched in bio-miR-21 pull-down in comparison with control pull-down.