An essential role for a mammalian SWI/SNF chromatin-remodeling complex during male meiosis

DEV073478 Supplementary Material

Files in this Data Supplement:

  • Supplemental Figure S1 -

    Fig. S1. BRM expression during spermatogenesis. BRM (red) expression analysis in testes from 4-week-old mice. Negative control (A) is free of nuclear staining of BRM. Meiotic cells are marked by γH2AX (green) in wild-type (B) and Brg1CKO (C) testis.

  • Supplemental Figure S2 -

    Fig. S2. Mvh-CRE is active in primordial germ cells. Immunohistochemistry of β-galactosidase protein in E15.5 XY gonads of Mvh-Cre; R26R/+. Round-shaped nuclei inside testis cords with diffuse DAPI staining identifies gonocytes (left panel, GCs: germ cells).

  • Supplemental Figure S3 -

    Fig. S3. Spermatogonia are present and proliferate normally in Brg1CKO testes. (A,B) Initial formation of spermatogonial cell population in wild-type and Brg1CKO testes at 7 dpp, depicted by pan-germ cell marker TRA98 (red). (C,D) Cell proliferation in 7 dpp wild-type and mutant testes. Phospho-histone H3 (green) marks actively dividing cells.

  • Supplemental Figure S4 -

    Fig. S4. The distribution of the different substages of meiotic prophase I was scored in 20 dpp wild-type and Brg1<B>CKO testes. Stages were classified by staining profiles of SYCP3 on nuclei spreads. Proportion of spermatocytes at various stages were significantly different (P<0.001, χ2 test).