Supplemental Data
Files in this Data Supplement:
- Supplemental Table S1, FigS1-8 (.pdf, 2.0 MB) - Table S1. Genotypes of the Offsprings obtained from the Tnrc6agt/+ X Tnrc6agt/+ or Tnrc6agt/+ X Tnrc6agt/gt Crosses. Fig.S1A-B) Mapping the integration site of gene trap vector in the intron 4 of GW182. Fig.S2. Tunnel staining of GW182 mutant and wild type embryo did not show significant differences in apoptosis. Fig.S3. A-B) Masson's trichrome staining of hematopietic progenitor cells from yolk sac of wild type and mutant embryos at 9.5dpc; C) Reduced expression of hemoglobin genes in the yolk ac of mutant mice as compared to wild type (Affymetrix array analysis, P<0.01 for all four hemoglobins). Fig.S4 Enrichment plots for targets of miRNAs significantly enriched in list of genes altered in GW182 mutant yolk sac. Fig.S5 Enrichment plots for targets of miRNAs that are not significantly enriched in list of genes altered in GW182 mutant yolk sac. Fig.S6 qRT-PCR confirmation of mRNA (A) and miRNA array data(B). Fig.S7 Increased expression of endothelial-cell specific genes and BMP-related genes in GW182 mutant yolk sac (Affymetrix array analysis, P<0.05 for all comparisons). Fig.S8. Expression of Tnrc6 family members in yolk sac.