van NieukerkenErik J.WagnerDavid L.BaldessariMarioMazzonLucaAngeliGinoGirolamiVicenzoDusoCarloDoorenweerdCamiel Antispila oinophylla new species (Lepidoptera, Heliozelidae), a new North American grapevine leafminer invading Italian vineyards: taxonomy, DNA barcodes and life cycle Zookeys22220122012170297710.3897/zookeys.170.2617 Antispila hydrangaeella http://species-id.net/wiki/Antispila_hydrangaeella ChambersFigs 3647Antispila hydrangaeella Chambers, 1874a: 170. Syntypes leafmines and larvae: [USA: Kentucky, Covington] on Hydrangea arborea [probably lost].Differential diagnosis.

DNA barcodes suggest that two species might be involved, and leafmines from a population in North Carolina (Smoky Mts NP) and northern Georgia do show some differences. Described adults and larvae are from the Georgia population.Externally, adult Antispila hydrangaeella (Fig. 36) is extremely similar to the other species of the Antispila ampelopsifoliella group, but it differs by the last six antennal segments being white and by genitalia and hostplant data. In male genitalia (Fig. 47) uncus only shallowly bilobed; valva with long pecten with more comb spines: ca. 20, triangular lobe absent, at base of valva beardlike setation; juxta rather wide, with groups of spines laterally; phallus with two very long terminal spines and many small spines near phallotrema, not forming a comb. Female genitalia not examined.

Biology.

Hostplant: Hydrangea arborea.

Leafmines.

One type (North Carolina) with long gallery mines, often following a vein, ending in a blotch with greenish to brown frass. The mines from Georgia with early gallery mine much contorted in a small area, with black frass, ending in elongate mine with blackish dispersed frass.

Distribution.

USA: Georgia, Illinois, Kentucky, North Carolina, presumably widespread in eastern United States.

Holocacista and Antispila adult habitus in dorsal or lateral(40, 41)view. 33 Holocacista rivillei, male, Italy 34 Antispila voraginella, male, USA: Arizona, genitalia slide EJvN3918 35 Antispila ampelopsifoliella, female, USA, Vermont: Salisbury, genitalia slide JCK15220 36 Antispila hydrangaeella, female, USA: Georgia, Chattahoochee NF 37 Antispila cf viticordifoliella, female, Canada: Ottawa 38, 39 Antispila cf isabella, male, upper and underside (39) with androconial scales, USA: Connecticut, Mansfield, DLW90J8 40 Antispila “vitis1”, female, USA: Florida, genitalia slide EJvN4205 41 Antispila cf viticordifoliella, female, USA: Florida, genitalia slide EJvN4207. Arrows indicate white tipped antennae in Antispila hydrangaeella and cf viticordifoliella.

Antispila species, male genitalia. 42–43 Antispila ampelopsifoliella, USA, New York state, genitalia slide EJvN4200 44–45 Antispila voraginella, USA, holotype, genitalia slide EJvN3916 46 Antispila cf isabella, USA: Kentucky, Morehead, genitalia slide CNC MIC1859 47 Antispila hydrangaeella, USA: North Carolina, NP Great Smoky Mts., genitalia slide EJvN4198.