Figure 2.

Effects of dietary sugars on expression of intestinal Ca²⁺ transporters in lactating and virgin rats. mRNA and protein expression levels were measured in the duodenum of lactating and virgin rats that had been fed a glucose (G), fructose (F), or starch (S) diet for 6 wk. A) mRNA level of TRPV6. B) mRNA and protein levels of CaBP9k (with β-actin as loading and transfer control for the Western blot). C) mRNA levels of PMCA1. D) mRNA levels of NCX1. mRNA levels were analyzed by real-time PCR using EF1α as a reference. Data were normalized to levels in virgin rats fed S. Attempts to determine protein levels of TRPV6, PMCA1, and NCX1 failed. Values represent means ± se (n=5–8/group). Differences (P<0.05) among means analyzed and presented as in Fig. 1.