Figure 6.

Effect of diet on histone H4 acetylation in the TRPV6 and CaBP9k promoter regions of lactating dams. ChIP assays were performed using anti-acetylated histone H4 antibody in the small intestine of lactating dams fed glucose, fructose, or starch diet for 6 wk. ChIP signals were detected by real-time RT-PCR using the primers for designated promoter regions of TRPV6 (A) and CaBP9k (B). ChIP signals were normalized to input signals. Bars are means ± se (n=3 or 4), with each n analyzed 2 times. Differences (P<0.05) among means within a region are indicated by differences in superscript letters, as described in Fig. 5.