Figure 9.
Effects of dietary sugar on expression of renal Ca2+ transporters in lactating and virgin rats. mRNA and protein expression levels were measured in the kidney homogenates of lactating dams and virgin rats fed a glucose (G), fructose (F), or starch (S) diet for 6 wk. A, B) mRNA levels of TRPV5 (A) and protein levels of CaBP28k (B). mRNA levels were normalized to EF1α mRNA levels, while β-actin was used as loading and transfer control in Western blots. C) mRNA levels of PMCA1. D) mRNA levels of NCX1. All data were normalized to levels in virgins fed S. Bars are means ± se (n=5–8/group). Differences (P<0.05) among means were analyzed and shown as described Fig. 1.