Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie01 - Movie 1: GFP-F-Tractin-P at the IS after engagement with a planar bilayer substrate. Recorded at 0.5 frames per second (fps) and played back at 15 fps. Related to Fig. 3.
  • Movie02 - Movie 2: GFP-F-Tractin-P at the IS after engagement with a coverslip substrate. Recorded at 0.5 fps and played back at 15 fps. Related to Fig. S3.
  • Movie03 - Movie 3: tdTomato-F-Tractin-P (left panel), GFP-myosin II-HC (center panel), and merged image of tdTomato-F-Tractin-P (green) and GFP-myosin II-HC (red) (right panel) at the IS after engagement with a planar bilayer substrate. All panels recorded at 0.25 fps and played back at 15 fps. Related to Fig. 3.
  • Movie04 - Movie 4: GFP-myosin II-HC at the IS after engagement with a planar bilayer substrate. Recorded at 0.4 fps and played back at 15 fps. Related to Fig. S3.
  • Movie05 - Movie 5: GFP-myosin II-RLC at the IS after engagement with a planar bilayer substrate. Recorded at 0.4 fps and played back at 15 fps. Related to Fig. S3.
  • Movie06 - Movie 6: Zoomed in video of TCR MCs (green) and GFP-F-Tractin-P (red) at the IS after engagement with a planar bilayer substrate. Recorded at 0.2 fps and played back at 15 fps. Related to Fig. 4.
  • Movie07 - Movie 7: TCR MCs (green) and GFP-myosin II-HC (red) at the IS after engagement with a planar bilayer substrate. Recorded at 0.4 fps and played back at 15 fps. Related to Fig. 4.
  • Movie08 - Movie 8: tdTomato-F-Tractin-P at the IS in a cell pre-incubated for 30 min with 50 �M BB and then engaged on planar bilayer substrate. Recorded at 0.2 fps and played back at 15 fps. Related to Fig. 5.
  • Movie09 - Movie 9: GFP-F-Tractin-P at the IS in a cell engaged on coverslip substrate, imaged for 2 min, and then treated with 0.2 �M CD. Recorded at 0.5 fps and played back at 15 fps. Related to Fig. 6.
  • Movie10 - Movie 10: GFP-F-Tractin-P at the IS in a cell engaged on coverslip substrate, imaged for 2 min, and then treated with 0.5 �M Jas. Recorded at 0.5 fps and played back at 15 fps. Related to Fig. 6.
  • Movie11 - Movie 11: GFP-F-Tractin-P at the IS in a cell engaged on coverslip substrate, imaged for 2 min, and then treated with 0.2 �M CD and 0.5 �M Jas. Recorded at 0.5 fps and played back at 15 fps. Related to Fig. 6
  • Movie12 - Movie 12: GFP-F-Tractin-P at the IS in a cell engaged on planar bilayer substrate, imaged for 2 min, and then treated with 0.2 �M CD and 0.5 �M Jas. Recorded at 0.4 fps and played back at 15 fps. Related to Fig. 7.
  • Movie13 - Movie 13: tdTomato-F-Tractin-P at the IS in a cell pre-incubated for 30 min with 50 �M BB, engaged on planar bilayer substrate, imaged for 2 min, and then treated with 0.2 �M CD, 0.5 �M Jas, and 50 �M BB. Recorded at 0.4 fps and played back at 15 fps. Related to Fig. 7
  • Movie14 - Movie 14: Alexa-647-labeled ICAM-1 at the IS in an untreated WT cell (left panel) and in a cell pre-incubated for 30 min with 50 �M BB (right panel). Both panels recorded at 0.4 fps and played back at 15 fps. Related to Fig. 8.