Files in this Data Supplement:
Table S1. Levels of biological material at 8 hpf. Levels of RNA (A), protein (B) and starting levels of total sulfation incorporation (C) comparable at 8 hpf.
Fig. S1. Adhesion defects in 2-OST MO are cell autonomous. (A-F) Single planes of z-stack at 4 hpf of mosaic embryos formed from injecting either control MO+lissamine-conjugated MO (B,C) or 2-OST MO+lissamine-conjugated MO (E,F) into a single cell at the 32-cell stage. Anti-β-catenin for control MO mosaic (A) or 2-OST MO (D). (G-I) Animal caps removed and immediately imaged from control MO (G), 2-OST MO (H), or 2-OST MO+2-OST RNA (I) embryos to examine adhesion defects. Minimal cells were observed around control MO and rescue-injected animal caps, but many cells were observed around 2-OST MO, indicating decreased adhesion.
Fig. S2. WT and control morphants exhibit comparable levels of β-catenin and E-cadherin. (A-F) WT levels are comparable to control MO embryos for β-catenin (A,C,D,F) and E-cadherin (B,C,E,F) in stabilized β-catenin rescue experiments as well as Wnt epistasis experiments.
Movie 1. 2-OST morphants exhibit failure to initiate epiboly. The WT embryo on the left undergoes normal epiboly and gastrulation whereas the 2-OST morphant embryo fails to initiate epiboly, eventually dying when the yolk explodes.
