The Ustilago maydis Nit2 Homolog Regulates Nitrogen Utilization and Is Required for Efficient Induction of Filamentous Growth

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Supplemental materials and methods, supplemental references, and supplemental table and figure legends.
    PDF file, 471K.
  • Supplemental file 2 - Full protein sequence alignment of NiT2 homologs.
    PDF file, 6.9MB.
  • Supplemental file 3 - Filament formation on Δton1 and Δncr1 on charcoal plates.
    PDF file, 916K.
  • Supplemental file 4 - Thin-layer chromatography of glycolipids isolated from SG200 and ΔN-Nit2 sporidia grown for 3 days on YNB nitrogen starvation medium.
    PDF file, 870K.
  • Supplemental file 5 - Genes commonly deregulated in Δncr1 and ΔN-Ncr1 sporidia 2 h after transfer from ammonium minimal medium to nitrogen starvation medium.
    MS Excel file, 74K.
  • Supplemental file 6 - Classification of genes regulated in both Δncr1 and ΔN-Ncr1 on nitrogen starvation medium into functional categories (FunCats).
    MS Excel file, 41K.
  • Supplemental file 7 - Genes commonly deregulated in both Δncr1 and Δton1 sporidia 2 h after transfer from ammonium minimal medium to nitrogen starvation medium.
    MS Excel file, 44K.
  • Supplemental file 8 - Classification of genes regulated in both Δncr1 and Δton1 on nitrogen starvation medium into functional categories (FunCats).
    MS Excel file, 40K.