Global Transcriptomic and Proteomic Responses of Dehalococcoides ethenogenes Strain 195 to Fixed Nitrogen Limitation

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - OpWise analysis of the microarray data to evaluate whether genes within a predicted operon were changing in the same direction (Fig. S1); transcriptomic and proteomic responses of genes in the gluconeogenesis pathway and citrate acid cycle (Fig. S2), in the purine metabolic pathway (Fig. S3), and in the pyrimidine metabolic pathway (Fig. S4).
    PDF file, 47K.
  • Supplemental file 2 - Detailed description of methods used in this study.
    PDF file, 44K.
  • Supplemental file 3 - Primer sequences used in this study for qPCR analysis (Table S1) and for RNA standards construction and RT-qPCR analysis (Table S2).
    PDF file, 29K.
  • Supplemental file 4 - List of all genes that had a false discovery rate (FDR) of less than 1% in the transcriptome (Table S3); genes that were upregulated (Table S4) or downregulated (Table S5) by two-fold or greater and had an FDR of less than 1% in the transcriptome; spectrum count of proteins detected in this study (Table S6).
    MS Excel file, 274K.
  • Supplemental file 5 - Enriched biological themes in the transcriptome (Table S7) and in the proteome (Table S8) according to the DAVID analysis.
    Zipped PDF file, 28K.
  • Supplemental file 6 - Overview of LC MS/MS proteomic data collected in this study (Table S9).
    Zipped PDF file, 12K.