Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS, and Its Role in Burkholderia glumae

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Table S1, primers used for RT-PCR
    Table S2, primers used for gel shift assays
    Table S3, identification of Usp2 under control of quorum sensing in Burkholderia glumae
    Table S4, annotation of B. glumae genes encoding Usps
    Table S5, identity among Usps in B. glumae
    Fig. S1, prediction of serine and threonine residues in B. glumae Usps
    Fig. S2, comparison of stress sensitivities of usp1 and usp2 mutants
    Fig. S3, swimming and swarming motilities of usp mutants of B. glumae
    Fig. S4, gel mobility shift assay using purified QsmR-His and a DNA fragment containing B. glumae usp regulatory regions
    Fig. S5, expression of rpoS is independent of quorum sensing
    Fig. S6, pathogenicity of usp1 and usp2 mutants
    supplemental description of materials and methods
    MS Word document, 2.5MB.