Bacterial Degradation of tert-Amyl Alcohol Proceeds via Hemiterpene 2-Methyl-3-Buten-2-ol by Employing the Tertiary Alcohol Desaturase Function of the Rieske Nonheme Mononuclear Iron Oxygenase MdpJ

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Supplemental description of materials and methods
  Fig. S1, degradation of TAME
  Fig. S2, degradation of TAEE
  Fig. S3, total ion chromatogram signals of samples from cells incubated on TAA
  Fig. S4, mass spectra of peaks 1 to 3 in Fig. S3 and most probable matches
  Fig. S5, GC-MS analysis after methyl ester derivatization
  Fig. S6, total ion chromatogram signals of samples from cells incubated on 3-methyl-3-pentanol
  Fig. S7, mass spectra of peak 1 in Fig. S6 and most probable match
  Fig. S8, possible mechanism for isomerization and dehydration of tertiary alcohols
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