Functional Identification of Two Novel Genes from Pseudomonas sp. Strain HZN6 Involved in the Catabolism of Nicotine

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Files in this Data Supplement:

• Supplemental file 1 - Oligonucleotide primers (Table S1); nicotine degradation and PN accumulation by strains N6mC8 and N6Δpao (Fig. S1); SDS-PAGE analysis of purified enzymes (Fig. S2); GC-MS mass spectrum of the methylamine after derivatization with pentafluorobenzaldehyde (Fig. S3); formation of hydrogen dioxide under both aerobic and anaerobic conditions (Fig. S4); HPLC analysis of the PNAO reaction and LC-MS mass spectrum of the SAP (Fig. S5); HPLC analysis of the coupled PNAO-SAPD reaction (Fig. S6).
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