Dynamics of Fos-Jun-NFAT1 complexes -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Ramirez-Carrozzi and Kerppola (2001) Proc. Natl. Acad. Sci. USA 98 (9), 4893–4898. (10.1073/pnas.091095998)

Supplemental Figure 5 Fig. 5.
Fluorescence resonance energy transfer analysis of Fos-Jun binding, reorientation and dissociation using single donor fluorophores on opposite ends of the oligonucleotide. The fluorescence emission spectra of a composite AP-1– NFAT site labeled with fluorescein (black), complexes formed by Fos- Jun_TR (magenta) and Fos- Jun_TR-NFAT1 (lime), and the dissociated components following incubation with genomic DNA (cyan) were compared between oligonucleotides labeled on the left (L) versus right (R) ends. The residual acceptor fluorescence of the dissociated complex was elicited by direct excitation.