Heterogeneity in Pseudomonas aeruginosa Biofilms Includes Expression of Ribosome Hibernation Factors in the Antibiotic-Tolerant Subpopulation and Hypoxia-Induced Stress Response in the Metabolically Active Population

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Table S1, microarray data showing relative mRNA levels for all P. aeruginosa PAO genes
    MS Excel file, 1.3MB.
  • Supplemental file 2 - Table S2, microarray data showing relative mRNA levels for selected housekeeping genes
    MS Excel file, 30K.
  • Supplemental file 3 - Table S3, microarray data showing relative mRNA levels for stress response genes
    MS Excel file, 29K.
  • Supplemental file 4 - Table S4, microarray data showing relative mRNA levels for selected genes regulated by quorum sensing and by the stationary-phase sigma factor rpoS
    MS Excel file, 30K.
  • Supplemental file 5 - Table S5, microarray data showing relative mRNA levels for ribosomal proteins, ribosome hibernation factors, and rRNAs
    MS Excel file, 34K.
  • Supplemental file 6 - Fig. S1, bioanalyzer results using picochip of total RNA extracted from P. aeruginosa biofilms
    Fig. S2, relative mRNA abundances from individual samples assayed by qRT-PCR and by Affymetrix microarrays
    Fig. S3, dynamics of induction of GFP expression in P. aeruginosa cultures and GFP fluorescence loss in P. aeruginosa cells
    Fig. S4, microarray results for mRNA amounts of genes regulated by the stationary-phase sigma factor rpoS and by quorum sensing
    Zipped PDF file, 421K.