Amino Acid Substitutions at Positions 122 and 145 of Hepatitis B Virus Surface Antigen (HBsAg) Determine the Antigenicity and Immunogenicity of HBsAg and Influence In Vivo HBsAg Clearance

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Table S1 (Expression plasmids for the expression of wt- and vtHVsAg.)
  Table S2 (Primers used for cloning or real-time PCR.)
  Fig. S1 (Reactivity of vtHBsAg to monoclonal anti-HBs antibody specific to HBsAg with G145R substitution.)
  Fig. S2 (Subcellular localization of wt- and vtHBsAgs with aa substitutions at position 145 or 122.)
  Fig. 3 (Kinetics of HBsAg and anti-HBs antibody response in preimmunized mice after HI.)
  Fig. 4 (Correlation of HBsAg and anti-HBs antibody in mouse sera.)
  Fig. 5 (Percentage of mice positive for HBsAg after challenge with pAAV-HBV-GB1.3.)
  Fig. 6 (VtHBsAgs with aa substitutions at position 145 or 122 retained the ability to induce HBsAg-specific CTL responses.)
  PDF file, 833K.