Elevated Carbon Dioxide Alters the Structure of Soil Microbial Communities

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Samples and their tagged primers used for amplifying 16S rRNA genes (Tables S1 and S2), the classified phylotypes detected at different taxonomical levels (Table S3), statistical analysis of differences in the microbial community compositions and structures between aCO2 and eCO2 at the community and phylum levels (Table S4), numbers of shared OTUs that were significantly changed at eCO2 based on the scaled abundances and unique OTUs detected only at aCO2 or eCO2 (Table S5), partial Mantel analysis of the relationship between the relative abundance of phyla (Table S6), orders (Table S7), families (Table S8), and genera (Table S9) and soil or plant properties, neighbor-joining tree of representative sequences of 16S rRNA genes of major phyla detected at aCO2 and eCO2 (Fig. S1), significantly changed OTUs in the phylum Actinobacteria at elevated CO2 using the response ratio method (Fig. S2), significantly changed microbial populations at the order levels in the phylum Proteobacteria (Fig. S3), significantly changed microbial populations at the class or lower levels in the phyla Acidobacteria, Verrucomicrobia, and Firmicutes (Fig. S4), flowchart of OTU identification processes (Fig. S5), and supplemental materials and methods.
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