Examination of Mechanism of N-Acetyl-1-d-myo-inosityl-2-amino-2-deoxy-α-d-glucopyranoside Deacetylase (MshB) Reveals Unexpected Role for Dynamic Tyrosine

Supplemental Data

Files in this Data Supplement:

• Supplemental Data (.pdf, 530 KB) - Kinetic plots (solvent viscosity effects, effect of NaCl on MshB activity, Michaelis-Menten plots for MshB mutants, NaF inhibition, and solvent isotope effects under V conditions, titration of D146A with Zn2+) and tables of solvent viscosity data are provided as supplemental data.