Linking osteopetrosis and pycnodysostosis: Regulation of cathepsin K expression by the microphthalmia transcription factor family -- Data Supplement - Supplemental Methods -- Proceedings of the National Academy of Sciences

Supplementary material for Motyckova et al. (May 1, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.091479298

Materials and Methods
RNA preparation and Northern Blots.
Primer sequences for probe construction were:

5' human cathepsin K cDNA primer, 5'-GACCCACAGGAAGCAATATAACAACAA;

3' human cathepsin K cDNA primer, 5'-GTAGCCCCCTCCACAGCCATCAT;

5' mouse cathepsin K cDNA primer, 5'-CTGCTACCCATGGTGAGCTTTGCTC;

3' mouse cathepsin K cDNA primer, 5'-GACAACTGACCCAATGTAGCACC;

5' mouse RANK cDNA primer, 5'-GCTTACCTGCCCAGTCTCATCGTTC;

3' mouse RANK cDNA primer, 5'-GGGCCGGTCCGTGTACTCATCCT;

5' human c-fms cDNA primer, 5'-GCGCCGCCATCCACCTCTAT;

3' human c-fms cDNA primer, 5'-TGCCGGCATGTTGGAAATCTACTT;

5' mouse c-fms cDNA primer, 5'-GACCTACCGTTGTACCGAGCTTGAAGACC;

3' mouse c-fms cDNA primer, 5'-GTAGGCATCTGCATGGACCGTGAGG;

5' mouse GAPDH cDNA primer, 5'-CATTGACCTCAACTACATGGTCTACATGTT;

3' mouse GAPDH cDNA primer, 5'-CTCTTGCTCAGAGGTCCACCACC;

5' human TRAP cDNA primer, 5'-GCGGCGGCCAGGGAGGACTAC; and

3' human TRAP cDNA primer, 5'-TGAGCCAGCCACAGAGCATAACACT.