Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Supplemental Movie 1. Typical NIH3T3 cell migration along a strip. The movie shows time-lapse recording of the cell shown in Figure 1A. Note the re-extension of the tail after retraction. Total recording time, 160 min.
- Movie02 - Supplemental Movie 2. Distinct behaviors of anterior and posterior regions during cell migration. Recording was performed with a motorized stage and a customized program that locks the position of the nucleus. Note the lack of discernible nuclear rotation as the cell migrates, and the re-extension of the tail soon after retraction. Total recording time, 60 min.
- Movie03 - Supplemental Movie 3. Differential functions of myosin II in anterior and posterior regions during cell migration. The movie shows the response of a cell treated globally with blebbistatin as described in Figure 1D. Note the striking stretching of the posterior region while the anterior region continues to migrate with little change in morphology. Total recording time, 8 hours.
- Movie04 - Supplemental Movie 4. Lack of responses to local application of Y-27632 in the anterior region. The movie shows time-lapse recording of the cell shown in Figure 2 A and B. Note that neither anterior movement nor tail retraction is affected by anterior Y-27632 treatment. Total recording time, 160 min.
- Movie05 - Supplemental Movie 5. Lack of responses to local application of blebbistatin in the tail region. The movie shows time-lapse recording of the cell shown in Figure 2 E. Note that tail retraction is not affected by local Y-27632 treatment in the tail region. Total recording time, 80 min.
- Movie06 - Supplemental Movie 6. Responses to local application of Y-27632 near the nucleus. The movie shows time-lapse recording of a cell similar to but different from the cell shown in Figure 2 C and D. Note the stretching of the region in front of a non-moving nucleus . Total recording time, 112 min.
- Movie07 - Supplemental Movie 7. Responses to local application of cytochalasin D in the anterior region. The movie shows time-lapse recording of the cell shown in Figure 3 A and B. Note the continuous movement of the nucleus despite the inhibition of frontal protrusion. Total recording time, 3 hours.
- Movie08 - Supplemental video 8. Responses to local application of cytochalasin D in the posterior region. The movie shows time-lapse recording of the cell shown in Figure 3 C and D. Note the reversal of nuclear movement. Total recording time, 3 hours.