Files in this Data Supplement:
Figure S1. The deep to superficial pattern of laminar specific markers is not altered in Sall1-/- animals. Tbr1 (red) staining of subplate and layer VI (*) cells in control (A) and Sall1-/- animals (B). Ctip2 (red) staining of layer VI and V (*) cells in control (C) and Sall1-/- (D) animals. Brn2 (red) staining of layer V and layer II/III cells (*) in control (E) and Sall1-/- (F) animals. Cux1 (red) staining of layer II/III (*) cells in control (G) and Sall1-/- (H) animals. Sections are counterstained with DAPI (blue). PC: progenitor population; IZ: intermediate zone; SP: subplate; CP: cortical plate; MZ: marginal zone. Scale bar (in H) represents: 75μm in A-H.
Figure S2. Sall1 regulates the number of cells committed to an early born fate. Birthdating studies of cells born on E11.5 (A,B), E12.5 (C,D), or E14.5 (E,F) (labeled cells are red) in the cortex at E18.5 in control (A,C,E) and Sall1-deficient (B,D,F) animals. Sections are counterstained with DAPI (blue). PC: progenitor population; IZ: intermediate zone; SP: subplate; CP: cortical plate; MZ: marginal zone. Scale bar (in F) represents: 75μm in A-F.
Figure S3. Alteration in the E14.5 rapidly exiting population at E15.5 in the absence of Sall. Immunohistochemistry staining of BrdU labeled cells. Pregnant animals were injected with BrdU at E14.5 and examined at E15.5. The E14.5 rapidly exiting population (bracketed population) was decreased in Sall1-/- animals (B) compared to controls (A) at E15.5. PC: progenitor population; IZ: intermediate zone. Scale bar (in B) represents: 75μm in A,B.
Figure S4. Sall1 regulates the number of IPCs during development. Tbr2 (red) staining at E14.5 (A,B) and E18.5 (C,D) in control (A,C) and Sall1-/- animals (B,D). The boundary between the PC/intermediate zone populations is indicated by the line (A-D). Sections are counterstained with DAPI (blue). PC: progenitor population. Scale bar (in D) represents: 75μm in A-D.
Figure S5. The cerebral cortex is decreased in size in Sall1-cKOEmx1 animals compared to controls at E18.5. Measurements of the surface area of the brain (top panel) and the length (middle panel) of control and Sall1-cKOEmx1 animals. Quantification of dorsal cell number in control and Sall1-cKOEmx1 animals (bottom panel). *, p<0.05; **, p<0.01, *** 0.001. MB: midbrain; CP: cortical plate; PC: progenitor population; IZ: intermediate zone; CP: cortical plate; MZ: marginal zone.
