Complex Physiology and Compound Stress Responses during Fermentation of Alkali-Pretreated Corn Stover Hydrolysate by an Escherichia coli Ethanologen

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Extracellular amino acid concentration in SynH and SynH supplemented with amino acids (Fig. S1); growth of the ethanologen in SynH and ACSH supplemented with protective osmolytes and amino acids (Fig. S2); expression of genes necessary for glycolysis, mixed acid fermentation, engineered ethanol production, and sugar transport during aerobic growth in GMM and during different phases of anaerobic growth in ACSH (Fig. S3); composition of ACSH, SynH, and GMM (Table S1); ions and metals present in ACSH and SynH and changes in concentration with respect to time and pH (Table S2); extracellular concentrations of carbon sources and metabolic end products during fermentation in SynH and SynH supplemented with amino acids (Table S3); extracellular concentrations of carbon sources and metabolic end products during fermentation in ACSH and ACSH supplemented with amino acids (Table S4); NMR analysis of ACSH at the beginning and end of fermentation (Table S5).
    PDF file, 421K.
  • Supplemental file 2 - Log2 expression values for all genes under all conditions (Table S6).
    MS Excel file, 4.2 MB.