Glyoxylate Metabolism Is a Key Feature of the Metabolic Degradation of 1,4-Dioxane by Pseudonocardia dioxanivorans Strain CB1190

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• Supplemental file 1 - Supplemental Materials and Methods (cloning of strain CB1190 genes, heterologous expression of strain CB1190 genes); SYBR Green and TaqMan primers used in qRT-PCR analyses (Table S1); homologous genes potentially encoding steps in the dioxane metabolic pathway of strain CB1190 (Table S2); genes differentially transcribed during growth of strain CB1190 with dioxane versus with pyruvate (Table S3) or with glycolate versus with pyruvate (Table S4); stable isotopic labeling of proteogenic amino acids from strain CB1190 cells grown with 1,4-U-¹³Cdioxane and either atmospheric (Table S5) or elevated (Table S6) CO₂; analysis of energy generation from strain CB1190 dioxane metabolite transformations (Table S7).
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