Versatile Transformation System That Is Applicable to both Multiple Transgene Expression and Gene Targeting for Thraustochytrids

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Schematic diagrams of EF-Neo^r, 18S-Ubi-Neo^r, and Ubi-EGFP (Fig. S1); growth inhibition of thraustochytrids by various antibiotics on PD agar plates (Fig. S2); sequence variation in T. aureum ATCC 34304 18S ribosomal DNA (Fig. S3); schematic diagrams of 18S ribosomal DNA-targeting vectors pTSCNeo^r, pTDCNeo^r, TSCNeo^r, and TDCNeo^r(Fig. S4); genomic PCR detecting Neo^r or egfp expression cassette in transformants generated by EF-Neor/Ubi-EGFP (Fig. S5); schematic diagrams showing two different modes of homologous recombination between the 18S ribosomal DNA-targeting vector and chromosomal locus of T. aureum ATCC 34304 (Fig. S6); genomic PCR for detection of tauΔ5des targeting generated by TauΔ5desKONeo^r and TauΔ5desKONeo^r/TauΔ5desKOHyg^r (Fig. S7).
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