Table 2.
Strains and plasmids used in the present study
| Strain or plasmid | Relevant characteristic | Source |
|---|---|---|
| Strains | ||
| A. flavus 3357-5 | A. flavus lacking pyrG | 8 |
| NRRL3357 | Standard A. flavus strain | 17 |
| BMU29791 | VRC-resistant A. flavus | Present study |
| AF-A | A. flavus 3357-5 transformed with plasmid pRG3-AMA1-NotI-A | Present study |
| AF-B | A. flavus 3357-5 transformed with plasmid pRG3-AMA1-NotI-B | Present study |
| AF-C | A. flavus 3357-5 transformed with plasmid pRG3-AMA1-NotI-C | Present study |
| AF-E | A. flavus 3357-5 transformed with plasmid pRG3-AMA1-NotI | Present study |
| AflavC | A. flavus NRRL3357 transformant with cyp51C gene from A. flavus BMU29791 | Present study |
| AflavC-3357 | A. flavus BMU29791 transformant with cyp51C gene from A. flavus NRRL3357 | Present study |
| AflavC-161 | A. flavus NRRL3357 transformant with T161C mutation in cyp51C gene | Present study |
| AflavC-788 | A. flavus NRRL3357 transformant with T788G mutation in cyp51C gene | Present study |
| AflavC-1325 | A. flavus NRRL3357 transformant with C1325A mutation in cyp51C gene | Present study |
| AflavC-1337 | A. flavus NRRL3357 transformant with A1337G mutation in cyp51C gene | Present study |
| E. coli DH10B | E. coli | Invitrogen |
| A. tumefaciens EHA105 | A. tumefaciens | 26 |
| Plasmids | ||
| pRG3-AMA1-NotI | Shuttle plasmid allowing autonomous nonintegrating replication of itself in life cycle of Aspergillus spp. | 16 |
| pRG3-AMA1-NotI-A | Recombinant plasmid of pRG3-AMA1-NotI with complete cyp51A gene of A. flavus | Present study |
| pRG3-AMA1-NotI-B | Recombinant plasmid of pRG3-AMA1-NotI with complete cyp51B gene of A. flavus | Present study |
| pRG3-AMA1-NotI-C | Recombinant plasmid of pRG3-AMA1-NotI with complete cyp51C gene of A. flavus | Present study |
| pDHt/sk | Binary plasmid | 26 |
| pDHt/sk-29791C | Recombinant plasmid of pDHt/sk with complete cyp51C gene of A. flavus BMU29791 | Present study |
| pDHt/sk-3357C | Recombinant plasmid of pDHt/sk with complete cyp51C gene of A. flavus NRRL3357 | Present study |
| pDHt/sk-3357C-161 | Recombinant plasmid of pDHt/sk with A. flavuscyp51C gene carrying T161C mutation | Present study |
| pDHt/sk-3357C-788 | Recombinant plasmid of pDHt/sk with A. flavuscyp51C gene carrying T788G mutation | Present study |
| pDHt/sk-3357C-1325 | Recombinant plasmid of pDHt/sk with A. flavuscyp51C gene carrying C1325A mutation | Present study |
| pDHt/sk-3357C-1337 | Recombinant plasmid of pDHt/sk with A. flavuscyp51C gene carrying A1337G mutation | Present study |
| pMD20-T vector | For T-A clone | TaKaRa Biotechnology |