Table 3.
MIC or MEC of antifungal drugs against Aspergillus sp. strainsl
| Strain | MIC/MEC (μg/ml) of: |
Portion of sequence of cyp51C gene containing mutationk | |||
|---|---|---|---|---|---|
| ITC | VRC | AMB | CASj | ||
| BMU29791a | 2 | 8 | 1 | 0.25 | ATGCGTGCTATCTAC (240A) |
| AflavCb | 2 | 8 | 1 | 0.25 | ATGCGTGCTATCTAC (240A) |
| AflavC-3357c | 1 | 0.25 | 1 | 0.25 | ATGCGTTCTATCTAC (240S) |
| AflavC-788d | 2 | 8 | 1 | 0.25 | ATGCGTGCTATCTAC (240A) |
| NRRL3357e | 1 | 0.25 | 1 | 0.25 | ATGCGTTCTATCTAC (240S) |
| AF-Af | ≥16 | 4 | 0.5 | 0.03 | NA |
| AF-Bg | ≥16 | 4 | 0.5 | 0.03 | NA |
| AF-Ch | ≥16 | 1 | 0.5 | 0.03 | NA |
| AF-Ei | 0.5 | 0.5 | 0.5 | 0.03 | NA |
VRC-resistant A. flavus strain isolated from the lung tissue of the patient.
A. flavus NRRL3357 transformant carrying the cyp51C gene of A. flavus BMU29791.
A. flavus BMU29791 transformant carrying the cyp51C gene of A. flavus NRRL3357.
A. flavus NRRL3357 transformant carrying the cyp51C gene with T788G missense mutation.
VRC-susceptible A. flavus strain obtained from the Fungal Genetics Stock Center.
A. flavus transformant with recombinant plasmid pRG3-AMA1-NotI-A.
A. flavus transformant with recombinant plasmid pRG3-AMA1-NotI-B.
A. flavus transformant with recombinant plasmid pRG3-AMA1-NotI-C.
A. flavus transformant with the empty plasmid pRG3-AMA1-NotI.
The antifungal activity of CAS was determined by using MEC.
Codons (underlined) and corresponding amino acid residues (in parentheses) are shown. NA, not available.
MEC, minimal effective concentration; ITC, itraconazole; VRC, voriconazole; AMB, amphotericin B; CAS, caspofungin.