A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Abeta -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Hashimoto et al. (2001) Proc. Natl. Acad. Sci. USA 98 (11), 6336–6341. (10.1073/pnas.101133498)

Supplemental Figure 10 Fig. 10.
Expression of HN poly(A)⁺ RNA in various tissues. (A) Expression of HN poly(A)⁺ RNA in human tissues. Membrane blotted with human tissue poly(A)⁺ RNA was probed with radiolabelled antisense HN (a), the 5' UTR (-432 to -414)-coding 19-mer antisense probe (b), or DT77 (c) (1, brain; 2, heart; 3, skeletal muscles; 4, colon; 5, thymus; 6, spleen; 7, kidney; 8, liver; 9, small intestine; 10, pancreas; 11, lung; 12, leukocytes). Bottom indicates the result of the hybridization of the same sheet with a b-actin probe (d). The numbers on the left side indicate the molecular sizes. In A-C, similar experiments were performed at least three times, and the representative result is shown. (B) Expression of HN poly(A)⁺ RNA in mouse tissues. The poly(A)⁺ RNA (2 µg per lane) extracted from various mouse organs was submitted to 1.2% agarose gel electrophoresis, blotted to nylon membrane, and hybridized with radiolabeled antisense HN (Upper) or the b-actin probe (Lower) (1, brain; 2, heart; 3, skeletal muscles; 4, thymus; 5, spleen; 6, kidney; 7, liver; 8, small intestine; 9, stomach; 10, skin; 11, lung). It is shown that the following points were different from the results with human tissues. First, murine skeletal muscles and liver contained fewer HN messages than did human tissues. However, these alterations in amounts appeared to be conditional, as skeletal muscles and liver obtained from some other mice contained abundant HN messages, while similar variation was not noted in other organs examined (data not shown). Second, the smaller message of 1 kb was expressed to a similar or even greater extent than that of 1.6 kb in the murine heart and kidney. In the murine brain, heart, and skeletal muscles, »0.4-kb message was additionally expressed, as observed in human heart and kidney. (C) Regional expression of HN poly(A)⁺ RNA in the human brain. Membrane blotted with human tissue poly(A)⁺ RNA from various regions in the brain (1, frontal lobe; 2, temporal lobe; 3, parietal lobe; 4, occipital lobe; 5, cerebellum; 6, lung) was hybridized with radiolabeled antisense HN (Upper) or a b-actin probe (Lower). To allow for quantitative comparison among membranes, the same amount of poly(A)⁺ RNA from lung was stained at lane 11 in A and lane 6 in C.