Supplementary material for Didier et al. (May 15, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.101126398

Fig. 6. (A) Glutamate iontophoretically applied on mitral cell dendrites revealed the presence of NMDA receptor not blocked by APV. Mitral cells were recorded with saline containing 1.3 mM Mg²⁺, 1 µM TTX, 20 µM BMI, 100 µM PTX, 100 µM APV, and 10 µM NBQX. (B) Glutamate iontophoretically applied on granule cell soma revealed the presence of NMDA receptor blocked by APV. Voltage-clamp recordings of pharmacologically isolated NMDA current in a granule cell recorded from a slice perfused with saline containing 1.3 mM Mg²⁺, 1 µM TTX, 20 µM BMI, 100 µM PTX, and 10 µM NBQX (holding potential, -60 mV).