Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs -- Data Supplement - Supplemental Figures -- Proceedings of the National Academy of Sciences

Supplementary material for Di Serio et al. (May 15, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.111423098

Fig. 7.
Southern blot analysis of genomic DNA (15 µg) extracted from untransformed tobacco (HAV425) and from TSC (TSC63) digested with the enzymes indicated. Fragments containing CHN sequences were visualized by using a ³²P-labeled CHN48 cDNA. Scale at left, size of markers in kb. A partial map of pBIBSCH10 used for transformation is shown with the positions of relevant restriction sites relative to the EcoRI site in parentheses. TSC contains three closely linked transgene loci, each containing at least one CHN48 transgene (SpeI digests). One T-DNA is missing » 600 nt from its left border (EcoRI digests). The truncated T-DNA is arranged in an incomplete inverted repeat with an intact T-DNA (BclI digests).