An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense -- Data Supplement - Supplemental Figure -- Proceedings of the National Academy of Sciences

Supplementary material for Xie et al. (May 15, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.111440998

Fig. 7.
Template dependency of inducible RNA-dependent RNA polymerase (RdRP) activity. Soluble protein extracts were prepared from salicylic acid (SA)-treated tobacco plants and passed through a DEAE column as described (1). The RdRP peak fractions were identified through assays of RdRP activity. The identified peak fractions were pooled and assayed for template dependency of the RdRP activity in absence of added RNA (lane 1) or presence of total RNA isolated from healthy untreated tobacco leaves (lane 2), SA-treated tobacco leaves (lane 3), tobacco mosaic virus (TMV)-infected tobacco leaves (lane 4), or RNA isolated from purified TMV (lane 5).

Reference:

1. Schiebel, W., Haas, B., Marinkovic, S., Klanner, A. & Sanger, H. L. (1993) J. Biol. Chem. 268, 11858-11867.