Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Supplementary movie 1 Dynamics of EGFP-LAMP3 granules in a control cells Cells were transfected with non-silencing siRNA and with a vector for the expression of the azurophilic granule marker EGFP-LAMP3. Images were obtained by TIRFM at 1 second intervals and recorded for 2 minutes. The video is shown at 6 fps.
- Movie02 - Supplementary Movie 2 Dynamics of EGFP-LAMP3 granules in a GMIP-downregulated cell. Cells were transfected with GMIP-silencing siRNA and with a vector for the expression of the azurophilic granule marker EGFP-LAMP3. Images were obtained by TIRFM at 1 second intervals and recorded for 2 minutes. The video is shown at 6 fps.
- Movie03 - Supplementary Movie 3 Actin remodeling and vesicular dynamics in an untreated HL-60 cell Cells were transfected with YFP-actin and DsRED-JFC1 and analyzed by TIRFM. Images were obtained by TIRFM at 1 second intervals and recorded for 1 minute. The video is shown at 10 frames per second (fps).
- Movie04 - Supplementary Movie 4 Actin remodeling and vesicular dynamics in a cytochalasin D-treated HL-60 cell. Cells were transfected with YFP-actin and DsRED-JFC1, treated with cytochalasin D for 30 min and analyzed by TIRFM. Images were obtained at 1 second intervals and recorded for 1 minute. The video is shown at 10 fps.